Compositions and methods comprising glycyl-tRNA synthetases having non-canonical biological activities
Isolated glycyl-tRNA synthetase polypeptides and polynucleotides having non-canonical biological activities are provided, as well as compositions and methods related thereto.
1. An isolated glycyl-tRNA synthetase (GRS) polypeptide of up to about 500 amino acids in length that comprises amino acid residues 367-438 of SEQ ID NO:1, or a GRS variant of up to about 500 amino acids in length that comprises a sequence at least 95% identical to residues 367-438 of SEQ ID NO:1.
2. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide comprises amino acid residues 367-438 of SEQ ID NO:1.
3. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide comprises amino acid residues 214-438, 214-685, 239-685, 265-685, 311-685, or 333-685 of SEQ ID NO:1, or where the GRS variant comprises a sequence at least 95% identical to residues 214-438, 214-685, 239-685, 265-685, 311-685, or 333-685 of SEQ ID NO:1.
4. The isolated GRS polypeptide of claim 3 , where the GRS polypeptide comprises amino acid residues 214-438, 214-685, 239-685, 265-685, 311-685, or 333-685 of SEQ ID NO:1.
5. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide or GRS variant is up to about 400 amino acids in length.
6. The isolated GRS polypeptide of claim 5 , where the GRS polypeptide comprises amino acid residues 367-438 of SEQ ID NO:1.
7. The isolated GRS polypeptide of claim 5 , where the GRS polypeptide comprises amino acid residues 214-438, 311-685, or 333-685 of SEQ ID NO:1, or where the GRS variant comprises a sequence at least 95% identical to residues 214-438, 311-685, or 333-685 of SEQ ID NO:1.
8. The isolated GRS polypeptide of claim 7 , where the GRS polypeptide comprises amino acid residues 214-438, 311-685, or 333-685 of SEQ ID NO:1.
9. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide or GRS variant is up to about 300 amino acids in length.
10. The isolated GRS polypeptide of claim 9 , where the GRS polypeptide comprises amino acid residues 367-438 of SEQ ID NO:1.
11. The isolated GRS polypeptide of claim 9 , where the GRS polypeptide comprises amino acid residues 214-438 of SEQ ID NO:1, or where the GRS variant comprises a sequence at least 95% identical to residues 214-438 of SEQ ID NO:1.
12. The isolated GRS polypeptide of claim 11 , where the GRS polypeptide comprises amino acid residues 214-438 of SEQ ID NO:1.
13. The isolated GRS polypeptide of claim 11 , where the GRS polypeptide consists essentially of amino acid residues 214-438 of SEQ ID NO:1.
14. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide or GRS variant is up to about 200 amino acids in length.
15. The isolated GRS polypeptide of claim 14 , where the GRS polypeptide comprises residues 367-438 of SEQ ID NO:1.
16. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide or GRS variant is up to about 100 amino acids in length.
17. The isolated GRS polypeptide of claim 16 , where the GRS polypeptide comprises residues 367-438 of SEQ ID NO:1.
18. The isolated GRS polypeptide of claim 16 , where the GRS polypeptide consists essentially of residues 367-438 of SEQ ID NO:1.
19. The isolated GRS polypeptide claim 1 , where the GRS polypeptide or GRS variant is pegylated.
20. The isolated GRS polypeptide of claim 1 , where the GRS polypeptide or GRS variant is fused to a heterologous fusion partner.
21. The isolated GRS polypeptide of claim 20 , where the heterologous fusion partner comprises an Fc region.
22. A dimeric or multimeric complex comprising at least one isolated GRS polypeptide or GRS variant of claim 1 .
23. A composition comprising a physiologically acceptable carrier and at least one component selected from the group consisting of: (i) an isolated glycyl-tRNA synthetase (GRS) polypeptide or GRS variant of claim 1 ; (ii) a fusion protein comprising a GRS polypeptide or GRS variant of (i) and a heterologous fusion partner; and (iii) a dimeric or multimeric complex comprising at least one isolated GRS polypeptide or GRS variant of (i).