Nanoparticles with covalently bound surfactant for drug delivery
The present invention concerns nanoparticles, compositions comprising the nanoparticles, methods for their production, and methods of using the nanoparticles for the delivery of biologically active agents (e.g., antibiotics or other drugs) to human or non-human subjects. In one embodiment, the nanoparticle is a “surfactant-free” nanoparticle in which the surfactant moiety is covalently attached to the backbone of the polymer.
1. A nanoparticle comprising an emulsified polymer, a surfactant, and a biologically active agent, wherein said biologically active agent and said surfactant are covalently attached to the polymer backbone of said polymer, and wherein the nanoparticle comprises no surfactant that is not covalently attached to the polymer backbone.
2. The nanoparticle of claim 1 , wherein the polymer is polyacrylate or polyacrylamide.
3. The nanoparticle of claim 1 , wherein the polymer is a co-polymer.
4. The nanoparticle of claim 1 , wherein the biologically active agent is a water-insoluble drug.
5. The nanoparticle of claim 1 , wherein the biologically active agent is an antibiotic.
6. The nanoparticle of claim 5 , wherein the antibiotic is an N-thiolated β-lactam antibiotic.
7. The nanoparticle of claim 1 , wherein the biologically active agent is covalently attached to the polymer backbone by an acryloyl amino acid.
8. A pharmaceutical composition comprising nanoparticles and a pharmaceutically acceptable carrier, wherein said nanoparticles comprise an emulsified polymer, a surfactant, and a biologically active agent, wherein said biologically active agent and said surfactant are covalently attached to the polymer backbone of said polymer and wherein said nano articles comprise no surfactant that is not covalently attached to the polymer backbone.
9. A method for producing a nanoparticle, comprising mixing a biologically active agent and a solution comprising monomers that have been pre-emulsified with a polymerizable surfactant; adding an initiator to the solution; and allowing the monomers to polymerize, wherein the resulting nanoparticle comprises an emulsified polymer, the surfactant, and the biologically active agent, wherein the biologically active agent and the surfactant are covalently attached to the polymer backbone of the polymer, and wherein the nanoparticle comprises no surfactant that is not covalently attached to the polymer backbone.
10. A method for producing a nanoparticle, comprising co-polymerizing a monomer, a biologically active agent, and a polymerizable surfactant wherein the biologically active agent and the surfactant are covalently attached to the polymer backbone of the resulting polymer, and wherein the nanoparticle comprises no surfactant that is not covalently attached to the polymer backbone.
11. The nanoparticle of claim 1 , wherein said surfactant is an acrylated surfactant.
12. The pharmaceutical composition of claim 8 , wherein said surfactant is an acrylated surfactant.
13. The method of claim 9 , wherein the polymerizable surfactant is an acrylated surfactant.
14. The method of claim 10 , wherein the polymerizable surfactant is an acrylated surfactant.
15. The pharmaceutical composition of claim 8 , wherein said biologically active agent is an antibiotic.
16. The method of claim 9 , wherein the biologically active agent is an antibiotic.
17. The method of claim 10 , wherein the biologically active agent is an antibiotic.