Bone-reinforcing food material
Disclosed is a bone-reinforcing agent which comprises a milk protein fraction having the following properties (1) to (4): (1) the milk protein fraction is derived from milk; (2) the milk protein fraction is a fraction containing a protein component having a molecular weight of 6,000 to 150,000 Daltons; (3) the milk protein fraction has a basic amino acid content of 12 to 14 wt % in the constitutional amino acid composition, and the ratio of the amount of a basic amino acid(s) to the amount of an acidic amino acid(s) is 0.5 to 0.7; and (4) the milk protein fraction has an activity of accelerating the calcification in an osteoblast.
1. A milk protein fraction degradation product obtained by degrading, with trypsin, chymotrypsin, or pepsin, a milk protein fraction having following characteristics (1) to (4): (1) the milk protein fraction is derived from milk, (2) the milk protein fraction comprises proteins and each protein in the milk fraction has a molecular weight of 6000 to 150,000 daltons determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), (3) when considered together, the proteins in the milk protein fraction are 12 to 14 percent of basic amino acids by weight of the constituent amino acid composition, and have a basic amino acid/acidic amino acid ratio of 0.5 to 0.7, and (4) the milk protein fraction has a promoting effect on calcification in an osteoblast.
2. A bone-reinforcing agent comprising the milk protein fraction degradation product according to claim 1 .
3. A bone-reinforcing pharmaceutical comprising the milk protein fraction degradation product according to claim 1 .
4. A bone-reinforcing food or drink comprising the milk protein fraction degradation product according to claim 1 .
5. A bone-reinforcing feed comprising the milk protein fraction degradation product according to claim 1 .
6. A method of reinforcing bone in a subject comprising orally administering to a subject a composition having the following characteristics (1) to (4):
(1) comprises components obtained from milk,
(2) comprises proteins, each of which has a molecular weight of 6000 to 150,000 daltons determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE),
(3) when considered together, the proteins in the composition are 12 to 14 percent of basic amino acids by weight of the constituent amino acid composition, and have a basic amino acid/acidic amino acid ratio of 0.5 to 0.7, and
(4) has a promoting effect on calcification in an osteoblast in vitro;
wherein the administration results in a reinforcing effect on bone in the subject.
7. A method of promoting calcification in osteoblasts comprising orally administering to a subject a composition having the following characteristics (1) to (4):
(1) comprises components obtained from milk,
(2) comprises proteins, each of which has a molecular weight of 6000 to 150,000 daltons determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE),
(3) when considered together, the proteins in the composition are 12 to 14 percent of basic amino acids by weight of the constituent amino acid composition, and have a basic amino acid/acidic amino acid ratio of 0.5 to 0.7, and
(4) has a promoting effect on calcification in an osteoblast in vitro;
wherein the administration has a promoting effect on calcification in osteoblasts in the subject.
8. The method according to claim 6 , wherein the composition is a milk protein fraction degradation product comprising a milk protein fraction having characteristics (1) to (4), degraded with a protease.
9. The method according to claim 6 , wherein the composition is a dry composition.
10. The method according to claim 9 , wherein the composition is a baked good.
11. The method according to claim 6 , wherein the composition is a liquid composition.
12. The method according to claim 11 , wherein the composition is a fruit juice.
13. The method according to claim 6 , comprising administering the composition in an amount of 1-50 mg/day.
14. The method according to claim 6 , comprising co-administering a calcium supplement with the composition.
15. The method according to claim 6 , wherein the subject is a person in recognized need of bone reinforcement.
16. The method according to claim 15 , wherein the subject is a person diagnosed with osteoporosis.
17. The method according to claim 15 , wherein the subject is a person at recognized risk of osteoporosis.