Production of lipidated proteins in
Production of a lipidated protein in an E. coli strain adapted to membrane protein expression.
1. A method of producing a lipidated protein in E. coli , comprising
providing a host E. coli cell transformed with an expression plasmid including a nucleotide sequence that encodes a naturally lipidated protein, wherein the host E. coli cell is C41 (DE3), C43 (DE3), DK8 (DE3)S, or C2014 (DE3); and the naturally lipidated protein has the amino acid sequence of SEQ ID NO: 1, and
culturing the host E. coli cell to express the naturally lipidated protein in lipidated form.
2. The method of claim 1 , wherein the host E. coli cell is C41 (DE3).
3. The method of claim 1 , wherein the host E. coli cell is C43 (DE3).
4. The method of claim 1 , further comprising, after the culturing step, isolating the naturally lipidated protein in lipidated form and confirming that the expressed protein is lipidated.
5. The method of claim 4 , wherein the host E. coli cell is C41 (DE3).
6. The method of claim 4 , wherein the host E. coli cell is C43 (DE3).