Identification of astrovirus VA1 associated with gastroenteritis in humans
Provided herein is a novel human astrovirus, its nucleic acid sequence, as well as methods to detect and diagnose the presence of the astrovirus.
1. An isolated nucleic acid molecule comprising a nucleotide sequence at least 95% identical to SEQ ID NO:1, or a complement to the entire said nucleotide sequence.
2. The isolated nucleic acid molecule of claim 1 wherein said nucleotide sequence is at least 98% identical to SEQ ID NO:1, or is a complement to the entire said nucleotide sequence.
3. The isolated nucleic acid molecule of claim 1 wherein said nucleotide sequence is identical to SEQ ID NO:1, or is a complement to the entire said nucleotide sequence.
4. A vector comprising the nucleotide sequence of claim 3 or a complement to the entire said nucleotide sequence.
5. An isolated cell comprising the vector of claim 4 .
6. A method to detect the presence of AstV-VA1 in a sample which method comprises contacting the sample with a probe that specifically hybridizes under stringent conditions to the nucleic acid molecule of claim 5 , or
which method comprises contacting the sample with amplification primers, amplifying nucleic acid in the sample, contacting the amplified nucleic acid with a probe that specifically hybridizes under stringent conditions to the nucleic acid molecule of claim 5 ,
wherein said stringent conditions are 6×SSC at about 45° C. followed by one or more washes of about 5-30 min each in 0.1×SSC, 0.1% SDS at 45-65° C.