Method of cultivating cell or tissue
A method for cultivating a culture of a cell, tissue, etc. There is provided a method of cultivating a culture including a cell or tissue (cell construct), imparting bending motion to the culture. By virtue of applying bending force to a culture of a cell, tissue, etc. (cell construct) to thereby curve the culture, continuous compression and extension in a direction of thickness from a concave portion toward a convex portion thereof are induced. The physical stimulation and deformation not attained by conventional pressurization, shear and tension, then can be loaded on the culture to thereby realize the culture appropriate for restoration of tissue at a region accompanied by bending.
1. A cultivating method of a culture including a cell and/or tissue, comprising:
putting the culture including the cell, the tissue or both thereof into a tube made from a semi-permeable membrane, through which a culture fluid can pass, and sealing the tube;
disposing a culture bed in a culture chamber which stores the culture fluid and in which the culture fluid is circulated, disposing the tube on a disposing part of the culture bed, the disposing part having elasticity so as to be curved by a load and be restored to its original form by cancellation of the load, and inserting the tube into each through hole in standing walls to hold the tube on the disposing part, the standing walls facing each other with the disposing part therebetween;
curving the disposing part by imparting the load to a back side of the culture bed to apply bending motion to the culture in the tube;
restoring the disposing part to its original form by canceling the load from the back side to apply the bending motion to the culture in the tube in a direction where the disposing part is restored; and
repeating the steps of said curving and restoring at least once, wherein the bending motion is applied to the culture in the tube periodically by restoration movement of the culture bed which results from the load to the culture bed and the cancellation of the load, and the culture fluid is supplied to the culture in the tube.
2. The culture method according to claim 1 , wherein the bending motion includes a process that brings the culture into a curving state.
3. The culture method according to claim 1 , wherein the bending motion is executed by the medium of the culture bed.
4. The culture method according to claim 1 , wherein the culture bed at its both ends is movably held, and the culture bed is curved by imparting the load to a center part of the culture bed.
5. The culture method according to claim 1 , wherein the bending motion is executed periodically or intermittently.
6. The culture method according to claim 1 , wherein the culture includes any of the cell, a cell scaffold, and an extracellular matrix produced by the cell.
7. The culture method according to claim 1 , wherein the culture is a three-dimensional culture scaffold where the cell is disseminated.
8. The culture method according to claim 1 , wherein the culture includes a gel substance.
9. The culture method according to claim 7 , wherein the three-dimensional culture scaffold is a bioabsorbable material.
10. The culture method according to claim 8 , wherein the gel substance is a bioabsorbable material.
11. The culture method according to claim 1 , wherein the bending motion is generated for the culture by any of
imparting continuous force to the culture, imparting intermittent force to the culture, or imparting continuous or intermittent force to the culture periodically.
12. The culture method according to claim 1 , further comprising pressuring the culture by pressuring the culture chamber, wherein pressure to the culture is given continuously or intermittently, or is changed periodically or irregularly.
13. The culture method according to claim 1 , wherein the bending motion is generated by imparting continuous force to the culture.
14. The culture method according to claim 1 , wherein the bending motion is generated by imparting intermittent force to the culture.