Non-toxic biofilm inhibitor
The present invention relates to a composition, device and method for preventing or inhibiting biofilm formation on biotic or abiotic surfaces. The composition comprises a peptide based on the C-terminal receptor binding domain of Pseudomonas aeruginosa type IV pilin, which binds to an abiotic surface (e.g., steel, plastic) with high affinity and prevents binding of a variety of P. aeruginosa strains to the surface. The inventive composition represents a non-toxic inhibitor for biofilm formation, particularly on an abiotic surface, which is responsible for a large number of problematic diseases and massive economic losses. The inventive method is useful as a safe and environmentally friendly means of modifying a surface of a variety of biomedical, nanotechnological, and biotechnological devices or articles.
1. A medical device having surfaces that are exposed to bacterial biofilm formation when the device is implanted in a body, in which such surfaces are coated with a synthetic pilin peptide having a core amino acid sequence of K/A/S/T-C -T/K/A-S/T-D/T/N-Q/V/A-D/E-E/P/A/N-Q/M/K-F/Y-I/T/R/L-P-K/N-G/T-C-S/D/T/Q/N-K/N/D/T (SEQ ID NO:2).
2. The device of claim 1 , wherein the synthetic pilin peptide coating the device surfaces has a core amino acid sequence selected from the group consisting of ID NOS:3, 4, 5, 6, 7, 13, 14, 15, 17, 18, and 19.
3. The device of claim 2 , wherein the synthetic pilin peptide coating the device surfaces has the core amino acid sequence of SEQ ID NO:3 or 4.
4. The device of claim 1 , wherein the coated surfaces are made of a material selected from the group consisting of stainless steel, tin, aluminum, titanium, chromium, plastic, glass, silicate, ceramics, and a mixtures thereof.
5. The device of claim 4 , wherein the coated surfaces are made of stainless steel.
6. The device of claim 4 , wherein the plastic is made of polyvinylchloride or polystyrene.