Manipulation of beads in droplets and methods for splitting droplets
The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
1. A method of splitting a droplet comprising a magnetically responsive bead in a droplet actuator, the method comprising:
(a) providing a droplet actuator comprising:
(i) a plurality of droplet operations electrodes configured to transport the droplet; and
(ii) one or more magnets to generate a magnetic field present at the plurality of droplet operations electrodes;
(b) providing a magnetically responsive bead-containing droplet on the droplet actuator;
(c) immobilizing the magnetically responsive bead using the magnetic field; and
(d) using the plurality of droplet operations electrodes to split the droplet into first and second droplets, wherein the magnetically responsive bead remains substantially immobilized.