Methods and compositions for the reduction of neutrophil influx and the treatment of bronchopulmonary displasia, respiratory distress syndrome, chronic lung disease, pulmonary fibrosis, asthma and chronic obstructive pulmonary disease
The present invention relates generally to the use of recombinant human CC10 (rhCC10), also known as recombinant human uteroglobin, for use as a therapeutic in the treatment of Respiratory Distress Syndrome (RDS), Bronchopulmonary dysplasia (BPD), chronic lung disease and/or pulmonary fibrosis, Asthma and Chronic Obstructive Pulmonary Disease (COPD). More particularly, the invention provides methods, including broadly the critical dosage ranges of rhCC10, which may be administered to safely and effectively treat the aforementioned conditions. The invention further provides a composition useful in administering rhCC10 to humans.
1. A method of reducing total protein concentration in the tracheal fluid of a patient in need thereof comprising: administering a therapeutically effective amount of rhCC10 to the patient,
wherein the rhCC10 is administered by the intratracheal, endotracheal, dialysate, ophthalmic, intravenous, systemic, or oral routes and total protein concentration in the patient's tracheal fluid is reduced.
2. The method of claim 1 wherein between about 15 ng to about 10 mg rhCC10 per kg of patient body mass is administered.
3. The method of claim 1 wherein between about 0.15 mg to about 5 mg rhCC10 per kg of patient body mass is administered.
4. The method of claim 1 wherein between about 1.5 mg and about 5 mg rhCC10 per kg of patient body mass is administered.
5. The method of claim 1 further comprising the step of measuring the patient's IL-8 serum levels wherein the patient's serum 1L-8 level is less than 120 pg/ml after administration.
6. The method of claim 1 further comprising the step of measuring the patient's serum or urine CC10 levels wherein the patient has a serum concentration of CC10 of about 100 ng/ml of serum to 2800 ng/ml of serum or a urine concentration of CC10 of about 100 ng/ml to about 10,000 ng/ml during treatment.
7. The method of claim 1 further comprising the step of measuring the patient's serum CC10 levels wherein the patient's peak serum concentration of CC10 is about 1280 ng/ml of serum to about 2800 ng/ml of serum after administration.
8. The method of claim 1 further comprising the step of measuring the patient's serum CC10 levels wherein a peak serum concentration of CC10 is reached within about 6 hours after initial dosage.
9. The method of claim 1 further comprising the step of measuring the patient's tracheal aspirate fluid CC10 levels wherein the patient reaches a peak tracheal aspirate fluid concentration of CC10 between about 12 and about 48 hours after initial dosage.
10. The method of claim 1 wherein the administration of rhCC10 is repeated at least once on a 48 hour interval.