Perlecan fragments as biomarkers of bone stromal lysis
A method for the detection in a body fluid of perlecan polypeptide fragments that are biomarkers of tumor metastasis, and antibodies for detecting these fragments are described. An immunoassay kit for detecting the presence of these biomarkers in a body fluid, such as serum or urine, is also described.
1. A method for detecting tumor cell metastasis in a human subject comprising the steps of:
(a) exposing a sample of body fluid from the subject to an antibody that is immunospecific for an antigen consisting of an amino acid sequence of SEQ ID NO:1, 2, 3, 4, or 5, wherein the body fluid is selected from the group consisting of blood plasma, serum and urine, whereby an antibody-antigen complex is formed;
(b) detecting the antibody-antigen complex;
(c) quantifying the amount of the detected antibody-antigen complex relative to a standard;
(d) determining the presence of the tumor cell metastasis in the subject based on a higher amount of the detected antibody-antigen complex relative to the standard; and
(e) selecting or devising treatment or counseling to the subject based on the presence of the tumor cell metastasis in the subject.
2. The method of claim 1 , wherein the body fluid is serum or urine.
3. The method of claim 1 , wherein the tumor cell metastasis is of a tumor type selected from the group consisting of prostate, breast, myeloma, bone, lung, pancreas, colon, liver, kidney, gastrointestinal tract, thyroid, uterus and ovary.