Methods for identifying an epitope of a polypeptide, chlamydial antigenic polypeptides identified thereby, and methods of use thereof
View Patent ↗The present invention provides a method of classifying an epitope displayed by a polypeptide; a method of determining the presence of an epitope on a polypeptide encoded by a test nucleotide sequence; and a method of generating a nucleotide sequence encoding a polypeptide that exhibits a selected epitope. The present invention provides antigenic polypeptides that display selected epitope(s); chimeric macromolecules comprising such polypeptides; and compositions comprising the antigenic polypeptides or chimeric macromolecules. The present invention further provides methods of inducing an immune response to a Chlamydia . The present invention further provides arrays of nucleic acids, arrays of polypeptides, and arrays of antibodies, which arrays are useful in identification and/or classification of a Chlamydia.
1. An isolated macromolecule comprising:
a) a heterologous bacterial protein; and
b) a polypeptide consisting of a sequence selected from:
(SEQ ID NO: 132)
CAETIFDVTTLNPTIAGAGDVKTSAEGC;
(SEQ ID NO: 43)
CAETILDVTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 23)
CTTLNPTIAGC;
(SEQ ID NO: 25)
CAETIFDVTTLNPTIAGC;
(SEQ ID NO: 26)
AETIFDVTTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 27)
CAETIFDVTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 28)
AETILDVTTLNPTIAG;
(SEQ ID NO: 29)
CAETILDVTTLNPTIAGC;
(SEQ ID NO: 30)
TTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 31)
CTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 32)
TTLNPTIAGAGDVKTSAE;
(SEQ ID NO: 33)
CTTLNPTIAGAGDVKTSAEC;
(SEQ ID NO: 34)
TTLNPTIAGKGTVVTSAE;
(SEQ ID NO: 35)
CTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 36)
TTLNPTIAGKGTVVSSAE;
(SEQ ID NO: 37)
CTTLNPTIAGKGTVVSSAEC;
(SEQ ID NO: 38)
TTLNPTIAGKGTVVASSAE;
(SEQ ID NO: 39)
CTTLNPTIAGKGTVVASSAEC;
(SEQ ID NO: 40)
TTLNPTTLNPTIAGKGTVVASSAE;
and
(SEQ ID NO: 42)
AETILDVTTLNPTIAGKGTVVTSAE.
2. An immunogenic composition comprising:
a) a macromolecule of claim 1 ; and
b) an adjuvant.
3. An isolated polypeptide consisting of a sequence selected from:
(SEQ ID NO: 132)
CAETIFDVTTLNPTIAGAGDVKTSAEGC;
(SEQ ID NO: 43)
CAETILDVTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 23)
CTTLNPTIAGC;
(SEQ ID NO: 25)
CAETIFDVTTLNPTIAGC;
(SEQ ID NO: 26)
AETIFDVTTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 27)
CAETIFDVTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 28)
AETILDVTTLNPTIAG;
(SEQ ID NO: 29)
CAETILDVTTLNPTIAGC;
(SEQ ID NO: 30)
TTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 31)
CTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 32)
TTLNPTIAGAGDVKTSAE;
(SEQ ID NO: 33)
CTTLNPTIAGAGDVKTSAEC;
(SEQ ID NO: 34)
TTLNPTIAGKGTVVTSAE;
(SEQ ID NO: 35)
CTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 36)
TTLNPTIAGKGTVVSSAE;
(SEQ ID NO: 37)
CTTLNPTIAGKGTVVSSAEC;
(SEQ ID NO: 38)
TTLNPTIAGKGTVVASSAE;
(SEQ ID NO: 39)
CTTLNPTIAGKGTVVASSAEC;
(SEQ ID NO: 40)
TTLNPTTLNPTIAGKGTVVASSAE;
and
(SEQ ID NO: 42)
AETILDVTTLNPTIAGKGTVVTSAE.
4. A composition comprising a polypeptide according to claim 3 .
5. An immunogenic composition comprising a polypeptide according to claim 3 .
6. An immunogenic composition comprising two or more different polypeptides, wherein each of the polypeptides consists of a sequence selected from:
(SEQ ID NO: 132)
CAETIFDVTTLNPTIAGAGDVKTSAEGC;
(SEQ ID NO: 43)
CAETILDVTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 23)
CTTLNPTIAGC;
(SEQ ID NO: 25)
CAETIFDVTTLNPTIAGC;
(SEQ ID NO: 26)
AETIFDVTTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 27)
CAETIFDVTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 28)
AETILDVTTLNPTIAG;
(SEQ ID NO: 29)
CAETILDVTTLNPTIAGC;
(SEQ ID NO: 30)
TTLNPTIAGAGCVKTSAEG;
(SEQ ID NO: 31)
CTTLNPTIAGAGCVKTSAEGC;
(SEQ ID NO: 32)
TTLNPTIAGAGDVKTSAE;
(SEQ ID NO: 33)
CTTLNPTIAGAGDVKTSAEC;
(SEQ ID NO: 34)
TTLNPTIAGKGTVVTSAE;
(SEQ ID NO: 35)
CTTLNPTIAGKGTVVTSAEC;
(SEQ ID NO: 36)
TTLNPTIAGKGTVVSSAE;
(SEQ ID NO: 37)
CTTLNPTIAGKGTVVSSAEC;
(SEQ ID NO: 38)
TTLNPTIAGKGTVVASSAE;
(SEQ ID NO: 39)
CTTLNPTIAGKGTVVASSAEC;
(SEQ ID NO: 40)
TTLNPTTLNPTIAGKGTVVASSAE;
and
(SEQ ID NO: 42)
AETILDVTTLNPTIAGKGTVVTSAE.