Multistep reaction lateral flow capillary
Disclosed is a lateral flow capillary device and uses thereof comprising a unipath bibulous capillary flow matrix and at least two reservoirs each in fluid communication with the capillary flow matrix wherein a reservoir contacts the capillary flow matrix through a passage having a rim pressing the matrix. The pressure that the rim applies on the matrix prevents leakage of liquids out of the capillary flow matrix at the reservoir/ matrix interface, allowing accurate sequential draining of liquid from the reservoirs. During use of the disclosed lateral flow capillary device a static interface is formed between the first liquid and the second liquid in an interface creation zone inside the capillary flow matrix wherein the first amount and second amount are such that first liquid substantially remains in the first reservoir and the second liquid substantially remains in the second reservoir subsequent to the formation of the static interface and wherein the interface begins to move only subsequent to exhaustion of a liquid from a reservoir.
1. A lateral flow capillary system, comprising:
a) a unipath bibulous capillary flow matrix having a thickness; and
b) at least two devices useful for preparation of said lateral flow capillary system engaged about said matrix, each of said devices comprising:
a first component comprising a single reservoir with at least one wall configured to hold liquids and a lowest area, said lowest area comprising a non-capillary opening comprising a hollow conduit with a rim and at least one extension protruding from an outer surface of said wall; and
a second component comprising a body with a counter-support platform at a top-end and at least one extension protruding from said body
wherein a said extension of said first component and a said extension of said second component are configured to mutually engage so that said rim and said counter-support platform are spaced apart by a distance and substantially parallel, wherein said distance is sufficient so that a said rim contacts said matrix when said first component and said second component are engaged about said matrix.
2. The system of claim 1 , wherein the bibulous capillary flow matrix is attached to a substantially impermeable backing material.
3. The system of claim 2 , wherein said backing material comprises polyethylene, polypropylene, poly(4-methylbutene), polystyrene, polymethylacrylate, poly(ethylene terephthalate), nylon, poly(vinyl butyrate), glass, ceramic, metal, polyurethane, latex, or silicone rubber.
4. The system of claim 1 , wherein the at least two devices useful for preparation of said lateral flow capillary device are adjacent.