Stabilized reverse micelle compositions and uses thereof
The invention relates to compositions and methods for drug delivery suitable for promoting the transmucosal absorption of drugs, especially drugs with poor intrinsic bioavailability, such as peptides, proteins, vaccines, and nucleic acids. The delivery system of this invention preferably comprises fatty acid esters and their hydrophilic derivatives that associate with water and other polar solvents to form reverse micelles that are physically stabilized in the presence of gastrointestinal fluid, water, and other hydrophilic solvents. Such stable reverse micelles are formed by suitable mixtures of polymeric or non-polymeric compounds with amphiphiles. Micelles made using these methods undergo phase transformation more slowly resulting in delayed drug release profiles and sustained absorption. When administered as a pharmaceutical to mucosal surfaces following oral ingestion or intranasal administration, therapeutic molecules principally solubilized in the aqueous phase are protected from digestion by mucosal enzymes and other mucosal degradative processes and are taken up by absorptive cell mechanisms and reach appropriate body compartments. The reverse micelle compositions may comprise mono-, di-glycerides and/or their transesterified products containing C6-C12 fatty acids chains, wherein the transester groups consist of hydrophilic moieties.
1. A reverse micelle composition which promotes absorption of a biologically active molecule by mucosal tissue comprising:
(a) a surfactant comprising one fatty acid ester, wherein the one fatty acid ester is between 6 and 12 carbon atoms in length, and further wherein the surfactant is a p-glycoprotein inhibitor;
(b) a hydrophilic phase, wherein the hydrophilic phase is present in an amount from about 0 to about 70% by weight;
(c) a biologically active molecule, wherein the biologically active molecule comprises at least one leutinizing hormone releasing hormone (LHRH) agonist, an analog thereof or LHRH; and
(d) a polymerized, water insoluble polymeric stabilizer, wherein the stabilizer is a natural polymer, a synthetic polymer or a mixture thereof.
2. Th reverse micelle composition of claim 1 , wherein the fatty acid ester is derivatized with acetic acid, citric acid, lactic acid, succinic acid, tartaric acid or mixtures thereof.
3. The reverse micelle composition of claim 1 , wherein the fatty acid ester is a caprylic acid of capric acid.
4. The reverse micelle composition of claim 1 , wherein the hydrophilic phase comprises, water, glycerol, sorbitol, mannitol, propylene glycol, ethylene glycol, polyethylene glycol or mixtures thereof.
5. The reverse micelle composition of claim 4 , wherein the hydrophilic phase further comprises a buffering agent, a tonicity agent, an oxidizing agent, a reducing agent, an antimicrobial agent, a preservative, a stabilizing agent, or a mixture thereof.
6. The reverse micelle composition of claim 1 , wherein the hydrophilic phase is present in an amount from about 0 to about 50% by weight.
7. The reverse micelle composition of claim 6 , wherein the hydrophilic phase is present in an amount from about 5 to 25% by weight.
8. The reverse micelle composition of claim 1 , wherein the fatty acid ester has an hydrophile-lipophile balance (HLB) of about 1 to about 20.
9. The reverse micelle composition of claim 8 , where the fatty acid ester has an HLB of about 5 to about 15.
10. The reverse micelle composition of claim 1 , wherein the LHRH agonist is leuprolide, goserelin, nafarelin or histrelin.
11. The reverse micelle composition of claim 1 , wherein the concentration of the biologically active molecule is more than 0.1 mg/ml soluble in the hydrophilic phase.
12. The reverse micelle composition of claim 11 , wherein the concentration of the biologically active molecule is more than 1 mg/ml soluble in the hydrophilic phase.
13. The reverse micelle composition of claim 1 , wherein the concentration of the biologically active molecule is from 0.05 to 100 mg/ml.
14. The reverse micelle composition of claim 1 , wherein the polymer is formed from a polymerizable fatty acid monomer.
15. The reverse micelle composition of claim 1 , wherein the polymer is formed by interfacial ionic polymerization with water or other initiators.
16. The reverse micelle composition of claim 1 , wherein the polymer is formed by condensation of cyanoacrylates, including alkylcyanoacrylates.
17. The reverse micelle composition of claim 1 , wherein the polymer is formed from condensation of ethyl 2-cyanoacrylate.
18. The reverse micelle composition of claim 1 , wherein the synthetic polymer is polylactide, polyglycolide, a mixture of polylactide and polyglycolide, polycaprolactone, polyortho esters, polyalkylenes, polyacrylamides, poly(hydroxy acids), polyanhydrides, polyorthoesters, polyacalate, polyvinyl alcohols, blends or copolymers thereof.