Highly sensitive system and method for analysis of troponin
The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.
1. A method for determining cardiovascular health of a human subject, comprising:
(a) determining a threshold concentration at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) in blood, serum or plasma in a healthy population, wherein the threshold concentration represents the 99 th percentile of cTnI or cTnT in the healthy population;
(b) measuring the blood, serum or plasma concentration of the at least one of cTnI and cTnT from the subject in an assay having a limit of detection for cTnI or cTnT of less than 3 pg/mL;
(c) determining cardiac damage in the subject when the concentration of the at least one of cTnI and cTnT in the sample is greater than the threshold concentration.
2. The method of claim 1 , wherein the threshold concentration is within the range of 1 pg/ml to 10 pg/ml.
3. The method of claim 1 , wherein the threshold concentration is less than about 7 pg/ml.
4. The method of claim 1 , wherein the threshold concentration is less than about 8 pg/ml.
5. The method of claim 1 , wherein the threshold concentration is less than about 9 pg/ml.
6. The method of claim 1 , wherein the threshold concentration is less than about 10 pg/ml.
7. The method of claim 1 , wherein the sample is taken during a stress test.
8. The method of claim 1 , wherein the sample is taken after a stress test.
9. The method of claim 1 , wherein the method detects single molecules of the at least one of cTnI and cTnT, or complexes or fragments thereof.
10. The method of claim 9 , comprising binding the at least one of cTnI and cTnT, or complexes or fragments thereof, with a label comprising a fluorescent moiety and detecting the label in a single molecule detector.
11. A method of evaluating a patient for the likelihood of a cardiac event in a human patient, comprising:
(b) detecting a concentration of cTnI or cTnT in a blood, serum or plasma sample from the patient in an assay having a limit of detection of less than 3 pg/mL; and
(c) determining the patient has an increased likelihood experiencing a cardiac event when the detected cTnI or cTnT concentration is greater than about 7 pg/ml.
12. The method of claim 11 , wherein the patient has an increased likelihood of experiencing a cardiac event when the detected level of cTnI or cTnT is greater than about 8 pg/ml.
13. The method of claim 11 , wherein the patient has an increased likelihood of experiencing a cardiac event when the detected level of cTnI or cTnT is greater than about 9 pg/ml.
14. The method of claim 11 , wherein the patient has an increased likelihood of experiencing a cardiac event when the detected level of cTnI or cTnT is greater than about 10 pg/ml.
15. A method for testing for myocardial damage in a human patient comprising, measuring the patient's blood, serum, or plasma concentration of cTnI or cTnT in an assay for cTnI or cTnT having a limit of detection of less than 10 pg/mL, determining mycardial damage in the patient when concentration is greater than the threshold concentration of cTnI or cTnT in blood, serum or plasma of a healthy human population, wherein the threshold concentration represents the 99 th percentile for cTnI or cTnT as measured in an assay having a limit of detection of less than 10 pg/ml.
16. The assay of claim 15 , wherein the assay is conducted within about five hours of admitting the patient to an emergency room with a clinical indication of myocardial infraction.
17. The method of claim 15 , wherein the method is performed periodically over a period of up to about 30 hours on samples from the same patient taken at different times during the 30 hour period.
18. A method of determining the progression of cardiac damage in an individual comprising: (i) obtaining a series of blood, serum or plasma samples from the individual, (ii) detecting a level of cTnI or cTnT in the series of samples in an assay having a limit of detection of less than 30 pg/mL; (iii) comparing the detected level of cTnI or cTnT in the series of samples to a predetermined threshold concentration of no more than about 10 pg/mL; and (iv) determining that the cardiac damage is resolving when the detected level of cTnI or cTnT is above the threshold level but decreases through the series of blood samples, and determining that cardiac damage is worsening when the detected level of cTnI is above the threshold level and increasing through the series of blood samples.
19. The method of claim 18 , wherein the threshold concentration is no more than about 7 pg/ml.
20. The method of claim 18 , wherein the threshold concentration is no more than about 8 pg/ml.
21. The method of claim 18 , wherein the threshold concentration is no more than about 9 pg/ml.