Culturing embryonic stem cells, embryonic stem-like cells, or induced pluripotent stem cells with a Muc1 or Muc1* ligand
The present application describes a method of culturing, expanding or growing stem or stem-like cells or induced pluripotent stem cells on a surface, including attaching the cells to the surface through a ligand that binds to the surface and the cells.
1. A method of culturing, expanding or growing embryonic stem cells, embryonic stem-like cells or induced pluripotent stem cells on a surface, comprising
a. exposing the embryonic stem cells, embryonic stem-like cells, or induced pluripotent stem cells to a surface coated with a Muc1 or Muc1* ligand;
b. adding medium, and
c. culturing the exposed cells,
wherein the cells remain undifferentiated.
2. The method according to claim 1 , wherein the surface is a membrane or a porous surface.
3. The method according to claim 1 , wherein the surface is further coated with extracellular matrix or a component of extracellular matrix.
4. The method according to claim 3 , wherein the component of extracellular matrix is RGD sequence containing peptides, poly-Lysine, collagen, laminin, Matrigel™ or Matrigel™-like substances.
5. The method according to claim 3 , wherein the extracellular matrix is Matrigel™ or Matrigel™-like substance.
6. The method according to claim 1 , wherein the ligand binds to the surface directly or indirectly through an intermediary.
7. The method according to claim 6 , wherein the intermediary is a chemical linker or another protein or a combination thereof.
8. The method according to claim 7 , wherein the protein is protein A or protein G.
9. The method according to claim 7 , wherein the linker is photo or chemically sensitive.
10. The method according to claim 6 , wherein the ligand or the intermediary is non-specifically adsorbed to the surface, or is covalently coupled or attached to the surface through an affinity tag-binding partner interaction.
11. The method according to claim 1 , wherein the ligand is linked to a polymer.
12. The method according to claim 1 , wherein the ligand is an antibody or a growth factor.
13. The method according to claim 12 , wherein the antibody specifically binds to PSMGFR or C-10 PSMGFR.
14. The method according to claim 12 , wherein the growth factor is wild-type NM23, or NM23-S120G mutant, or bFGF.
15. The method according to claim 1 , wherein the surface is not Matrigel™.
16. The method according to claim 1 , wherein the cell is cultured without fibroblast feeder cells.
17. The method according to claim 1 , wherein the cell is removed from the surface without manual dissection.
18. A method of culturing, expanding or growing embryonic stem cells, embryonic stem-like cells or induced pluripotent stem cells on a surface, comprising
a. exposing the embryonic stem cells, embryonic stem-like cells or induced pluripotent stem cells to a surface coated with an extracellular matrix a component of extracellular matrix;
b. adding medium containing a Muc1 or Muc1* ligand; and
c. culturing the exposed cells,
wherein the cells remain undifferentiated.
19. The method according to claim 18 , wherein the agent is an antibody.
20. The method according to claim 18 , wherein the agent is wild-type NM23 or NM23-S120G mutant.
21. The method according to claim 18 , wherein the surface is not Matrigel™.
22. The method according to claim 18 , wherein the cell is cultured without fibroblast feeder cells.
23. The method according to claim 18 , wherein the cell is removed from the surface without manual dissection.
24. The method according to claim 18 , wherein the surface is a membrane or a porous surface.
25. The method according to claim 18 , wherein the component of extracellular matrix is RGD sequence containing peptides, poly-Lysine, collagen or laminin.
26. The method according to claim 18 , wherein the extracellular matrix is Matrigel™ or Matrigel™-like substance.
27. A method of culturing, expanding or growing embryonic stem cells, embryonic stem-like cells or induced pluripotent stem cells on a surface, comprising
culturing the embryonic stem cells, embryonic stem-like cells or induced pluripotent stem cells in MUC1*-positive cell conditioned media, wherein the cells remain undifferentiated.
28. The method according to claim 27 , wherein the MUC1*-positive cells are cancer cells.
29. The method according to claim 28 , wherein the MUC1*-positive cells are selected from T47D, ZR-75-30, or ZR-75-1.
30. The method according to claim 27 , wherein the surface is a membrane or a porous surface.
31. The method according to claim 27 , wherein the surface is coated with an extracellular matrix or a component of extracellular matrix.
32. The method according to claim 31 , wherein the component of extracellular matrix is RGD sequence containing peptides, poly-Lysine, collagen or laminin.
33. The method according to claim 31 , wherein the extracellular matrix is Matrigel™ or Matrigel™-like substance.
34. The method according to claim 27 , wherein the surface is not Matrigel™.
35. The method according to claim 27 , wherein the cell is cultured without fibroblast feeder cells.
36. The method according to claim 27 , wherein the cell is removed from the surface without manual dissection.