Method for cancer detection, diagnosis and prognosis
The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.
1. A system for isolating a circulating tumor cell, said system comprising:
a parylene microfilter device comprising a membrane filter consisting of a parylene substrate having an array of holes with a predetermined shape and dimension, wherein the holes on the membrane filter have a rectangular shape each with a dimension of 6 μm by about 40 μm; and
a constant pressure delivery system coupled to the parylene microfilter device for maintaining a constant pressure, wherein the isolated circulating tumor cells are isolated with at least 90% efficiency and with an enrichment of at least 200-fold, and wherein the isolated circulating tumor cells have at least a 90% cell viability.
2. The system of claim 1 , wherein the pressure is between 0.01 to 0.3 psi.
3. The system of claim 2 , wherein the pressure is between 0.1 to 0.3 psi.
4. The system of claim 1 , wherein the array of holes is between 1 and 40,000 per square millimeter.
5. The system of claim 4 , wherein the array of holes is between 5,000 and 40,000 per square millimeter.
6. The system of claim 1 , wherein the array of holes is between 1 and 850 per square millimeter.
7. The system of claim 1 , wherein the membrane filter has a figure of merit up to 890%/hr.
8. The system of claim 1 , wherein the thickness of the membrane is at least about 1 μm.