Vault complexes for facilitating biomolecule delivery
The invention relates to compositions of vault complexes containing recombinant membrane lytic proteins, such as an adenovirus protein VI lytic domain, and methods of using the vault complexes to facilitate delivery and entry of a biomolecule into a cell or subject.
1. A vault-like particle comprising a membrane lytic domain comprising the amino acid sequence of SEQ ID NO:3, a major vault protein comprising the amino acid sequence of SEQ ID NO:16, and an antibody binding Z domain.
2. The vault-like particle of claim 1 , wherein the membrane lytic domain is fused to the C-terminus of the major vault protein, and the antibody binding Z domain is fused to the N-terminus of the major vault protein, forming a fusion protein.
3. The vault-like particle of claim 1 , wherein said fusion protein comprises the amino acid sequence of SEQ ID NO:11.