Vaccine for modulating between T1 and T2 immune responses
The present invention provides liposomal vaccines containing immunogenic lipopeptides that are capable of modulating the humoral and cellular immune responses in vivo.
1. A liposomal composition comprising a MUC1 dilipopeptide, said dilipopeptide comprising TSAPDTRPAPGS (amino acids 24-35 of SEQ ID NO:1), wherein two amino acids of said dilipopeptide are lipidated and wherein the dilipopeptide is (a) a dilipopeptide selected from the group consisting of BP1-265 (SEQ ID NO:14), BP1-271 (SEQ ID NO:15), BP1-273 (SEQ ID NO:17), BP1-274 (SEQ ID NO:18), and BP1-236 (SEQ ID NO:13), or (b) a dilipopeptide differing from (a) solely by the replacement of at least one lipid group with another lipid group.
2. The composition of claim 1 wherein the dilipopeptide differs from (a) solely by the replacement of at least one lipid group with another lipid group and in which each lipidated amino acid is lipidated with a lipid selected independently from the group consisting of myristyl, palmitoyl and lauryl.
3. The composition of claim 1 , which further comprises an adjuvant.
4. The composition of claim 3 , wherein the adjuvant is selected from the group consisting of lipid A or a derivative thereof, monophosphoryl lipid A, muramyl dipeptide, and IL-2.
5. The composition of claim 3 , wherein the adjuvant is lipid A or a derivative thereof or monophosphoryl lipid A.
6. The composition of claim 1 , wherein the dilipopeptide comprises one or more glycosylated amino acids.
7. The composition of claim 1 , wherein the dilipopeptide further comprises a MUC 1 carbohydrate linked to serine or threonine of said dilipopeptide.
8. The composition of claim 1 , wherein the dilipopeptide is BP1-265 (SEQ ID NO:14).
9. The composition of claim 1 , wherein the dilipopeptide is BP1-271 (SEQ ID NO:15).
10. The composition of claim 1 , wherein the dilipopeptide is BP1-273 (SEQ ID NO:17).
11. The composition of claim 1 , wherein the dilipopeptide is BP1-274 (SEQ ID NO:18).
12. The composition of claim 1 , wherein the dilipopeptide is BP1-236 (SEQ ID NO:13).
13. A liposomal composition comprising a MUC1 dilipopeptide, said dilipopeptide comprising TSAPDTRPAPGS (amino acids 24-35 of SEQ ID NO:1), wherein two amino acids of said dilipopeptide are lipidated and said dilipopeptide comprises BP1-265 (SEQ ID NO:14).