Mammalian milk microorganisms, compositions containing them and their use for the treatment of mastitis
The present invention relates to a novel approach to prevent or treat mastitis both in humans and animals through the use of mammal milk-derived microorganisms obtained from healthy hosts of the homologous species; to those new probiotic microorganisms obtained from milk able to reduce infectious mastitis and the method of screening used for their obtaining; to the use of these probiotic bacteria for the prophylaxis or treatment against mastitis and other diseases; and finally, to compositions comprising these compounds.
1. A probiotic strain or a mixture of strains consisting of lactic acid bacteria or bifidobacteria strains present in the fresh milk from a mammalian species selected in lactic acid culture media, wherein said probiotic strain or mixture of strains (i) are capable of being transferred to the mammary gland after oral intake and/or colonise the mammary gland after its topical application, (ii) are able to reduce the rates of survival and/or the rates of adhesion to epithelial cells of Staphylococcus aureus , and (iii) capable of protecting animals from mastitis, characterised in that the strain is selected from the group consisting of Bifidobacterium breve deposited in the CECT under Accession No. 7263 , Bifidobacterium breve deposited in the CECT under Accession No. 7264 , Lactobacillus reuteri deposited in the CECT under Accession No. 7260 , Lactobacillus plantarum deposited in the CECT under Accession No. 7262 , Lactobacillus fennenturn in the CECT under Accession No. 7265 and Lactobacillus reuteri deposited in the CECT under Accession No. 7266 , Enterococcus hirae deposited in the CECT under Accession No. 7410 , Lactobacillus plantarum deposited in the CECT under Accession No. 7412 , Enterococcus faecalis deposited in the CECT under Accession No. 7411 , Lactobacillus salivarius deposited in the CECT under Accession No. 7409 , Lactobacillus reuteri deposited in the CECT under Accession No. 7413.
2. A pharmaceutical composition comprising at least a probiotic strain according to claim 1 .
3. The composition as defined in claim 2 which is in a frozen, lyophilized or dried form.
4. The composition as defined in claim 2 wherein the probiotic strain or the mixture thereof is in a partially or totally inactivated form.
5. A method of treating mastitis, said method comprising administering an effective amount of the probiotic strain of claim 1 to a subject or animal in need thereof.
6. The method according to claim 5 , wherein the strain is administered orally, topically, nasally, enterally, ocularly, urogenitally, rectally, or vaginally.
7. A method of treating neonatal diarrhea , said method comprising administering an effective amount of the probiotic strain of claim 1 to a subject or animal in need thereof.
8. The method according to claim 7 , wherein the strain is administered to a lactating woman or animal for the therapeutic or prophylactic treatment of the foetus and/or their breastfed babies or pups.
9. The method according to claim 8 , wherein the strain is administered to the lactating woman or animal orally, topically, nasally, enterally, ocularly, urogenitally, rectally, or vaginally.
10. The method according to claim 7 , wherein the strain is administered orally, topically, nasally, enterally, ocularly, urogenitally, rectally, or vaginally.