Apparatus and method for micromanipulation of microscale objects using laser light delivered through a single optical fiber and axicon lens
A single optical fiber having a distal end is optically coupled to the laser and distilling terminated with an axicon lens optically coupled to the single optical fiber to form a microscopic distal tip to provide a spatially shaped elongated laser focused spot for microprocessing and/or microdissection of a microscale object. A pulsed or continuous laser beam or superposition of pulsed and continuous laser beams is generated, controllably spatially shaped, selectively oriented, selectively moved via movement of a single optical fiber terminated with the axicon lens, and the oriented, spatially shaped laser beam applied via the single optical fiber terminated with the axicon lens to a living or nonliving microscopic object for manipulation, micro-dissection, alteration/ablation, and excitation of the living or nonliving microscopic object.
1. An apparatus for use with a laser comprising:
a single optical fiber having a distal end and being optically coupled to the laser;
a rotational stage coupled to the distal tip of the single optical fiber, a pulsed NIR laser and a continuous wave UV or NIR laser for producing an elongated beam both of which are optically coupled to the single optical fiber, wherein orientation of the elongated beam is controlled by the rotational stage;
an excitation source of light for fluorescent excitation combined with the pulsed NIR laser and a continuous wave UV or NIR laser; and
an axicon lens optically coupled to and terminating the single optical fiber to form a microscopic distal tip to provide a spatially shaped elongated laser focused spot for microprocessing and/or microdissection of a microscale object.
2. The apparatus of claim 1 where the axicon lens is integrally formed in the distal end of the single optical fiber.
3. The apparatus of claim 1 where the axicon lens is discretely formed apart from the distal end of the single optical fiber.
4. The apparatus of claim 1 where the axicon lens is arranged and configured to provide a linear profile to the spatially shaped elongated laser focused spot.
5. The apparatus of claim 1 wherein the axicon lens has a focal plane and the focused spot has a length, and further comprising a variable aperture disposed at the focal plane of the axicon lens in order to control the length of the focused spot.
6. The apparatus of claim 1 where the axicon lens has a predetermined cone angle selected at an angular cone angle to provide optical trapping and binding in the far field as well as near-field of the distal tip of the single optical fiber.
7. The apparatus of claim 6 where the predetermined cone angle is approximately 30° or less.
8. The apparatus of claim 6 where the predetermined cone angle is approximately 90°.