Methods for determining response to neoadjuvant therapy and survival using MicroRNA-10b
The present invention provides methods for determining response to neoadjuvant therapy and metastasis-free survival in pancreatic ductal adenocarcinoma based upon the level of microRNA expression and optionally the presence of a protein cancer cell marker in biological samples such as formalin-fixed paraffin-embedded specimens using in situ hybridization and optionally an immunohistochemical assay.
1. A method for determining response to neoadjuvant therapy in pancreatic ductal adenocarcinoma (PDAC) comprising
(a) obtaining a biological sample from a patient with PDAC;
(b) determining the level of microRNA-10b in the biological sample with a microRNA-10b specific probe having the sequence 5′-CA+CAA+ATT+CGG+TT+CTA+CAG+GGTA-3′ (SEQ ID NO:3), wherein +N denotes a locked nucleic acid modified nucleotide;
(c) comparing the level of microRNA-10b in the biological sample to control samples expressing relatively low, medium or high levels of microRNA-10b; and
(d) diagnosing response of the patient to neoadjuvant therapy, wherein a level of microRNA-10b expression in the biological sample comparable to the control sample expressing a relatively low level of microRNA-10b indicates that the patient is likely to respond to neoadjuvant therapy.
2. A method for determining response to neoadjuvant therapy in pancreatic ductal adenocarcinoma (PDAC) comprising
(a) obtaining a biological sample from a patient with PDAC;
(b) detecting cytokeratin or mucin cancer cell marker-positive cells of the biological sample;
(c) determining the level of microRNA-10b in the cytokeratin or mucin cancer cell marker-positive cells;
(d) comparing the level of microRNA-10b in the cytokeratin or mucin cancer cell marker-positive cells to control cells expressing relatively low, medium or high levels of microRNA-10b; and
(e) diagnosing response of the patient to neoadjuvant therapy, wherein a level of microRNA-10b expression in the cytokeratin or mucin cancer cell marker-positive cells comparable to the control sample expressing a relatively low level of microRNA-10b indicates that the patient is likely to respond to neoadjuvant therapy.
3. A method for determining metastasis-free survival in pancreatic ductal adenocarcinoma (PDAC) comprising
(a) obtaining a biological sample from a patient with PDAC;
(b) determining the level of microRNA-10b in the biological sample with a microRNA-10b specific probe having the sequence 5′-CA+CAA+ATT+CGG+TT+CTA+CAG+GGTA-3′ (SEQ ID NO:3), wherein +N denotes a locked nucleic acid modified nucleotide;
(c) comparing the level of microRNA-10b in the biological sample to control samples expressing relatively low, medium or high levels of microRNA-10b; and
(d) diagnosing metastasis-free survival of the patient, wherein a level of microRNA-10b expression in the biological sample comparable to the control sample expressing a relatively low level of microRNA-10b indicates that the patient has an increase in metastasis-free survival.
4. A method for determining metastasis-free survival in pancreatic ductal adenocarcinoma (PDAC) comprising
(a) obtaining a biological sample from a patient with PDAC;
(b) detecting cytokeratin or mucin cancer cell marker-positive cells of the biological sample;
(c) determining the level of microRNA-10b in the cytokeratin or mucin cancer cell marker-positive cells;
(d) comparing the level of microRNA-10b in the cytokeratin or mucin cancer cell marker-positive cells to control cells expressing relatively low, medium or high levels of microRNA-10b; and
(e) diagnosing metastasis-free survival of the patient, wherein a level of microRNA-10b expression in the biological sample comparable to the control sample expressing a relatively low level of microRNA-10b indicates that the patient has an increase in metastasis-free survival.