Granted Patent
B2
US 8,597,945 · App. 13/406,206 · Granted Dec 3, 2013
Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
Assignee:
Wisconsin Alumni Research Foundation
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Abstract
A method of differentiating embryonic stem cells into ventral spinal progenitor cells is disclosed. In one embodiment, the invention comprises culturing a population of cells comprising a majority of cells that are characterized by an early rosette morphology and are Sox1 − /Pax6 + in the presence of retinoic acid, wherein the cells express Hoxb4, but not Otx2 or Bf1.
Claims (1)
1. An isolated population of human ventral spinal progenitor cells wherein at least 80% of cells within the population are human ventral spinal progenitor cells and are Hoxb4 + , HoxC8 + , Olig2 + , Nkx2.2 + , Irx3 + , Otx2 − , Pax7 − and Phox2b − .
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 4, 2014
From: ZHANG, SU-CHUN; LI, XUE-JUN
To: WISCONSIN ALUMNI RESEARCH FOUNDATION
Reel/Frame 032132/0477 →
CONFIRMATORY LICENSE
Recorded Mar 5, 2012
From: WISCONSIN ALUMNI RESEARCH FOUNDATION
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 027802/0445 →
Continuity (7)
Provisional Application
60498831
· Aug 29, 2003
Provisional Application
60499570
· Sep 2, 2003