Method for producing foods from culture of
View Patent ↗A method for producing a food product containing nattokinase but containing little or none vitamin K2, comprising treating a Bacillus natto culture or culture supernatant with at least one coagulant selected from the group consisting of an inorganic coagulant and a cationic polymer coagulant (excluding chitosan). As the inorganic coagulant, calcium chloride or poly aluminum chloride is preferable, and their mixtures with a phosphorus coagulant are also preferably used. As the cationic polymer coagulant, cationic polysaccharides, hexamethylenediamine/ephichlorohydrin polycondensates or dimethylamine/epichlorohydrin polycondensates are preferably used, and their mixtures with a polyacrylate are also preferably used.
1. A method for producing a food product from Bacillus natto culture, said food product containing nattokinase and about 1 μg or less of vitamin K2/g dry weight, comprising:
treating a Bacillus natto culture or culture supernatant with an inorganic coagulant in combination with a phosphorus-containing coagulant, wherein the inorganic coagulant is calcium chloride and the phosphorus-containing coagulant is at least one selected from the group consisting of disodium hydrogenphosphate, sodium dihydrogenphosphate, potassium dihydrogenphosphate and tripotassium phosphate, and
filtrating the Bacillus natto culture or culture supernatant and the inorganic coagulant in combination with the phosphorus-containing coagulant, thereby removing vitamin K2 from the Bacillus natto culture or culture supernatant while nattokinase is retained in the Bacillus natto culture or culture supernatant.
2. The method of claim 1 , wherein the food product is in a form of one selected from the group consisting of concentrate, paste, powder, granule, capsule, drinkable preparation and tablet.