DNA fragment having promoter function
View Patent ↗The present invention relates to a method of inducing expression of a promoter function of various genes in a Coryneform bacterium related to function exertion, in order to exert the function of a Coryneform bacterium highly and effectively under an anaerobic condition, for producing an organic compound useful under an anaerobic condition, more particularly, provides a method of enhancing and/or suppressing the promoter function related to various genes, for the purpose of highly and effectively expressing various protein genes necessary for production of an objective substance, and suppressing expression of an unnecessary protein gene. The DNA fragment of the present invention is useful as a primer which is introduced into a transformed Coryneform bacterium producing a useful substance such as lactic acid and succinic acid highly and at a high efficiency.
1. A vector comprising a DNA fragment comprising SEQ ID NO:32 and a heterologous gene.
2. A transformant of a Coryneform bacterium transformed with the vector of claim 1 , wherein the vector is inserted in a plasmid or a chromosome upstream of a gene encoding an enzyme involved in metabolism in the Coryneform bacterium, and wherein the plasmid or chromosome can autonomously-replicate in the Coryneform bacterium.
3. The transformant according to claim 2 , wherein the enzyme is at least one enzyme or coenzyme involved in a glycolysis pathway, a reductive tricarboxylic acid pathway, an anaplerotic pathway, an amino acid synthesis pathway, a purine synthesis pathway, a pyrimidine synthesis pathway, a cholesterol synthesis pathway, a fatty acid synthesis pathway, or a pathway derived from one or more of these pathways.