Methods and compositions for cell culture platform
The present invention provides a synthetic cell culture platform, comprising: a two dimensional and/or three dimensional surface comprising peptides conjugated to said surface and methods of using the platform for increasing cell adhesion, stable attachment and/or proliferation of cells grown on the platform and for promoting differentiation of neural stem cells into neurons.
1. A cell culture platform, comprising: a two dimensional and/or three dimensional surface comprising peptides conjugated to said surface, wherein said peptides comprise the amino acid sequence CCRRIKVAVWLC (SEQ ID NO: 1).
2. The cell culture platform of claim 1 , wherein the two dimensional surface is an in vitro cell culturing surface.
3. The cell culture platform of claim 1 , wherein the three dimensional surface is a hydrogel.
4. The cell culture platform of claim 1 , wherein the peptides are conjugated to said surface at a concentration of 10 μM or less.
5. A method of increasing cell adhesion, stable attachment and/or proliferation of cells on two dimensional and/or three dimensional surfaces, comprising:
contacting the cells with the platform of claim 1 ; and
culturing the cells, wherein the cultured cells have increased cell adhesion, stable attachment and proliferation as compared to a control.
6. The method of claim 5 , wherein the cells are stem cells.
7. The method of claim 6 , wherein the stem cells are neural stem cells or neural progenitor cells (NPCs).
8. The method of claim 7 , wherein the neural stem cells or neural progenitor cells are human.
9. A method of promoting differentiation of neural stem cells or neural progenitor cells into neurons, comprising: contacting neural stem cells or neural progenitor cells with the platform of claim 1 ; and culturing the cells on said platform, thereby promoting the differentiation of the cells into neurons.
10. The method of claim 9 , wherein the neural stem cells or neural progenitor cells are human.