Methods and compositions for improved F-18 labeling of proteins, peptides and other molecules
The present application discloses compositions and methods of synthesis and use of 68 Ga, 18 F or 19 F labeled molecules of use in PET or MRI imaging. Preferably, the 18 F or 19 F is conjugated to a targeting molecule by formation of a complex with a group IIIA metal and binding of the complex to a chelating moiety, which may be directly or indirectly attached to the targeting molecule. In other embodiments, the 68 Ga, 18 F or 19 F labeled moiety may comprise a targetable construct used in combination with a bispecific antibody to target a disease-associated antigen. In more preferred embodiments, a chelating moiety or targetable construct may be conjugated to a targeting molecule, such as an antibody or antibody fragment.
1. A composition comprising a targetable construct, wherein the targetable construct is conjugated to a chelating moiety attached to metal- 18 F or metal- 19 F, wherein the metal is selected from the group consisting of aluminum, gallium, indium, lutetium and thallium.
2. The composition of claim 1 , wherein the targetable construct is a peptide.
3. The composition of claim 2 , wherein the targetable construct is selected from the group consisting of IMP 448, IMP 449, IMP 460, IMP 461, IMP 462, IMP 465, IMP 466, IMP 467, IMP 468, IMP 469, IMP 470, IMP 471, IMP 473, IMP 479, IMP 485, IMP 486 and IMP 487.
4. The composition of claim 1 , wherein the chelating moiety is selected from the group consisting of DOTA, TETA, NOTA, NODA, (tert-Butyl) 2 NODA, NODA-MPAA, NETA, C-NETA, L-NETA, and S-NETA.
5. The composition of claim 4 , wherein the chelating moiety is selected from the group consisting of wherein ITC is isothiocyanate, NODA-GA, NODA-GA(tButyl) 3 , Di-t-butyl-NOTA, NOTA-thiosemicarbazide, NODA-MPAA, and NODA-MPAEM.
6. The composition of claim 1 , wherein the metal is aluminum.
7. A composition comprising a targetable construct, wherein the targetable construct is conjugated to a chelating moiety, wherein the chelating moiety is attached to 68 Ga, wherein the targetable construct is selected from the group consisting of IMP 448, IMP 449, IMP 460, IMP 461, IMP 462, IMP 465, IMP 466, IMP 467, IMP 468, IMP 469, IMP 470, IMP 471, IMP 473, IMP 479, IMP 485, IMP 486 and IMP 487.
8. The composition of claim 7 , wherein the chelating moiety is selected from the group consisting of DOTA, TETA, NOTA, NODA, (tert-Butyl) 2 NODA, NODA-MPAA, NETA, C-NETA, L-NETA, and S-NETA.
9. The composition of claim 7 , wherein the chelating moiety is selected from the group consisting of wherein ITC is isothiocyanate, NODA-GA, NODA-GA(tButyl) 3 , Di-t-butyl-NOTA, NOTA-thiosemicarbazide, NODA-MPAA, and NODA-MPAEMan.