Volume exclusion agent to enhance formation of extracellular matrix
A method of enhancing the formation of extracellular matrix in culture. Cells in culture secrete most of the collagen into the media as unprocessed procollagen, i.e., the cells do not convert procollagen to collagen. In contrast, normal extracellular matrix deposition involves procollagen processing to collagen, fibril assembly and deposition into the cell layer to form a collagenous extracellular matrix. The addition of certain growth factors and the addition of a thin layer of a certain volume exclusion agent on top of the cells dramatically enhances the conversion of procollagen to collagen and will increase the amount of collagen and extracellular matrix associated with the cells. This invention advances bioengineering of connective tissues for medical applications that require an extensive and functional extracellular matrix with high tensile strength such as those in the cornea stroma, skin, tendons, ligaments, articular cartilage and the intervertebral disks.
1. A method of enhancing the formation of extracellular matrices in culture comprising the steps of:
plating out fibroblast cells on a tissue culture substrate;
adding media containing a collagen synthesis stimulating growth factor to the plated cells;
incubating the plated cells to facilitate attachment of the cells to the tissue culture substrate;
removing the media from the substrate containing the growth factor stimulated cells;
adding a volume exclusion agent capable of converting procollagen to collagen on top of the plated fibroblast cells wherein the volume exclusion agent is a hydrogel wherein the hydrogel creates a semipermeable barrier on the top of the plated fibroblast cells;
adding tissue culture media containing the collagen synthesis stimulating growth factor on top of the hydrogel; and
incubating the plated fibroblast cells in a tissue culture incubator;
whereby after incubation procollagen is converted to collagen and extracellular matrices are formed.
2. The method according to claim 1 , wherein the volume exclusion agent is an agarose overlay.
3. The method according to claim 2 , wherein the agarose overlay is between a 1%-6% solution of low melting temperature agarose in distilled water mixed with an equal volume of media containing the collagen synthesis stimulating growth factor.
4. The method according to claim 1 wherein the fibroblast cells are selected from the group consisting of keratocytes, and tenocytes.
5. A method of enhancing the formation of extracellular collagen matrices in culture by increasing the conversion of procollagen to collagen comprising the steps of:
plating out keratocytes on a tissue culture substrate;
adding a volume exclusion agent capable of converting procollagen to collagen on top of the plated keratocytes wherein the volume exclusion agent is a hydrogel wherein the hydrogel creates a semipermeable barrier on the top of the plated keratocytes;
adding tissue culture media containing a collagen synthesis stimulating growth factor to the substrate containing the overlaid plated keratocytes; and
incubating the plated keratocytes in a tissue culture incubator
whereby after incubation procollagen is converted to collagen and extracellular collagen matrices are formed.
6. The method according to claim 5 , wherein the volume exclusion agent is an agarose overlay.
7. The method according to claim 6 , wherein the agarose overlay is between a 1%-6% solution of low melting temperature agarose in distilled water.
8. A method of enhancing the formation of extracellular matrices in culture by increasing the conversion of procollagen to collagen comprising the steps of:
plating out keratocytes on a tissue culture substrate;
adding media containing a collagen synthesis stimulating growth factor to the plated keratocytes;
incubating the plated keratocytes to facilitate attachment of the keratocytes to the tissue culture substrate;
removing the media from the substrate containing the growth factor stimulated keratocytes;
adding a hydrogel overlay capable of converting procollagen to collagen on top of the plated keratocytes wherein the hydrogel creates a semipermeable barrier on the top of the plated keratocytes;
adding tissue culture media containing the growth factor to the substrate containing the overlaid plated keratocytes; and
incubating the plated keratocytes in a tissue culture incubator;
whereby after incubation procollagen is converted to collagen and extracellular collagen matrices are formed.
9. The method according to claim 8 , wherein the growth factor is insulin.
10. The method of claim 1 , wherein the growth factor is selected from the group consisting of IGF-I, PDGF, TGF-β and insulin.
11. The method of claim 1 , wherein the volume exclusion agent is a hydrogel selected from the group consisting of agarose, kappa-carrageenan, iota-carrageenan, gelatin, elastin-mimetic protein polymers and silk-elastin block copolymers.
12. The method of claim 5 , wherein the growth factor is selected from the group consisting of IGF-I, PDGF, TGF-β and insulin.
13. The method of claim 5 , wherein the volume exclusion agent is a hydrogel selected from the group consisting of agarose, kappa-carrageenan, iota-carrageenan, gelatin, elastin-mimetic protein polymers and silk-elastin block copolymers.
14. The method of claim 8 , wherein the growth factor is selected from the group consisting of IGF-I, PDGF, TGF-β and insulin.
15. The method of claim 8 , wherein the volume exclusion agent is a hydrogel selected from the group consisting of agarose, kappa-carrageenan, iota-carrageenan, gelatin, elastin-mimetic protein polymers and silk-elastin block copolymers.