Affinity peptides toward BMP-2
We have disclosed affinity peptides toward BMP-2. More specifically we have disclosed an affinity biomatrix where the affinity peptide is covalently attached to a biocompatible, biodegradable polymer. The affinity biomatrix is useful in preparing controlled release devices for BMP-2.
1. An isolated affinity peptide toward bone morphogenic protein-2 (BMP-2) selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10.
2. An affinity biomatrix comprising a biocompatible, biodegradable polymer and at least one isolated affinity peptide toward bone morphogenic protein-2 (BMP-2) selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10, wherein the at least one affinity peptide toward BMP-2 is covalently attached to the biocompatible, biodegradable polymer.
3. The affinity biomatrix of claim 2 , wherein the biocompatible, biodegradable polymer is a natural polymer, wherein the natural polymer is selected from the group consisting of collagen, elastin, keratin, silk, polysaccharides, glucosaminoglycans (GAGs), and combinations thereof.
4. The affinity biomatrix of claim 3 , wherein the natural polymer is collagen.
5. A controlled release device comprising:
a) an affinity biomatrix comprising a biocompatible, biodegradable polymer and at least one isolated affinity peptide toward bone morphogenic protein-2 (BMP-2) selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10, wherein the at least one affinity peptide toward BMP-2 is covalently attached to the biocompatible, biodegradable polymer; and
b) BMP-2.
6. The controlled release device of claim 5 , wherein the device is in a form selected from the group consisting of sponges, particles, injectable gels, injectable liquids, membranes, films, fibers and fiber based scaffolds.
7. A kit comprising:
a) an affinity biomatrix comprising a biocompatible, biodegradable polymer and at least one isolated affinity peptide toward bone morphogenic protein-2 (BMP-2) selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10, wherein the at least one affinity peptide toward BMP-2 is covalently attached to the biocompatible, biodegradable polymer; and
b) BMP-2.