Self-compatible, rapid-cycling
The invention provides Brassica rapa plants and seeds thereof that are self-compatible, rapid-cycling and lack inbreeding depression. For instance, the invention provides plants and seeds of the Brassica rapa line designated B3. The invention thus relates to plants, seeds and tissue cultures of Brassica rapa plants that are self-compatible, rapid-cycling and lack inbreeding depression, such as Brassica rapa line B3, and methods to produce and propagate said plants by crossing such a Brassica rapa plant with itself, or another Brassica rapa plant. The invention further relates to seeds and plants produced by such crossing. Educational materials, such as a kit comprising said Brassica rapa plants are also provided by the invention.
1. A Brassica rapa plant that is self-compatible, rapid-cycling and lacks inbreeding depression wherein the plant is a plant of line B3 or a progeny thereof; wherein a sample of said line B3 has been deposited under ATCC Accession No. PTA-9873.
2. The plant of claim 1 , wherein the plant is inbred.
3. A plant part of the plant of claim 1 .
4. The plant part of claim 3 , wherein said part is selected from the group consisting of a protoplast, ovule, cell, pollen grain, embryo, cotyledon, hypocotyl, meristem, root, pistil, anther, flower, stem, pod or petiole.
5. A tissue culture of regenerable cells of a plant of claim 1 .
6. The tissue culture according to claim 5 , comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, shoots, pistil, flower, seed and stem.
7. A method of propagating the plant of claim 1 , comprising crossing the plant with itself or a second plant.
8. A method of vegetatively propagating a plant of claim 1 comprising:
(a) collecting tissue capable of being propagated from a plant of claim 1 ;
(b) cultivating said tissue to obtain proliferated shoots; and
(c) rooting said proliferated shoots to obtain rooted plantlets.
9. The method of claim 8 , further comprising growing plants from said rooted plantlets.
10. A method of producing a mutant allele comprising
(a) obtaining a plant of claim 1 or tissue thereof;
(b) subjecting said plant or tissue thereof to mutagenesis; and
(c) recovering a plant comprising a mutant allele.
11. The method of claim 10 , wherein mutagenesis comprises use of a mutagen selected from the group consisting of gamma irradiation and chemical mutagenesis.
12. A method of producing a self-compatible, rapid-cycling, Brassica rapa plant lacking inbreeding depression comprising:
(a) crossing a plant of claim 1 with a second plant to produce progeny plants; and
(b) selecting at least a first progeny plant from step (a) that comprises a desired trait.
13. The method of claim 12 , further comprising:
(c) backcrossing the selected first progeny plant from step (b) with a plant of claim 1 ; and
(d) selecting at least a first backcross progeny plant from step (c) that comprises the desired trait.
14. The method of claim 12 , wherein the desired trait is selected from the group consisting of a pigmentation-defective mutant trait and developmental mutant trait.
15. The method of claim 12 , wherein the desired trait is selected from the group consisting of embryo lethal, albino, leaf shape, leaf number, leaf color, variegated leaf, flower shape, flower number, flower color, seed color, seed shape, tissue- or organ specific pigmentation, a photosynthesis trait, internode shape, hypocotyl shape, cotyledon shape, stem shape, pod shape, dwarf and over-sized.
16. The method of claim 12 , wherein the desired trait is due to a mutation in a gene encoding a protein involved in a biochemical or developmental pathway.
17. An educational kit comprising the plant of claim 1 or a seed that produces said plant.
18. A Brassica rapa plant of line B3, further comprising a single locus conversion, wherein the plant otherwise comprises essentially all of the physiological and morphological characteristics of Brassica rapa line B3, and wherein a sample of seed Brassica rapa line B3 has been deposited under ATCC Accession No. PTA-9873.