Method for measuring carbon nanotubes taken-up by a plurality of living cells
The present invention provides methods, apparatuses and kits for determining the presence and the concentration of nanoparticles in a given area, solution or region via cellular uptake and/or adsorption monitored through laboratory equipment. For example, the present invention provides a method of quantifying one or more nanoparticles by incubating a nanoparticle solution comprising one or more nanoparticles with one or more cells; isolating the one or more cells; lysing the one or more cells to release a cell lysate; separating the cell lysate electrophoretically on a gel; digitizing the gel to form a gel image; quantifying the nanoparticle intensity in the gel image; and correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
1. A method of quantifying one or more nanoparticles comprising the steps of:
lysing one or more cells thought to comprise one or more nanoparticles to release a cell lysate;
separating the cell lysate electrophoretically on a gel;
digitizing the gel to form a gel image;
quantifying a nanoparticle intensity of one or more regions in the gel image; and
correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
2. The method of claim 1 , wherein the one or more nanoparticles comprise fullerenes, quantum dots, inorganic nanospheres, metallic nanospheres, nanorods, carbon nanotubes, Si-nanotubes, nanowires, single-wall nanotubes, multi-wall nanotubes and combinations thereof.
3. A method of isolating one or more nanoparticles comprising the steps of:
incubating a nanoparticle solution comprising one or more nanoparticles with one or more cells;
isolating the one or more cells;
lysing the one or more cells to release a cell lysate;
separating the cell lysate electrophoretically on a gel;
digitizing the gel to form a gel image; quantifying the nanoparticle intensity in the gel image; and
correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
4. The method of claim 3 , wherein the one or more nanoparticles comprise fullerenes, quantum dots, inorganic nanospheres, metallic nanospheres, nanorods, carbon nanotubes, nanowires, single-wall nanotubes, multi-wall nanotubes and combinations thereof.
5. A method of determining the uptake and/or adsorption of nanoparticles by a cell comprising the steps of:
incubating a nanoparticle solution comprising one or more nanoparticles with one or more cells;
isolating the one or more cells;
lysing the one or more cells to release a cell lysate;
separating the cell lysate electrophoretically on a gel;
digitizing the gel to form a gel image;
quantifying the nanoparticle intensity in the gel image; and
correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
6. The method of claim 5 , wherein the one or more nanoparticles comprise fullerenes, quantum dots, inorganic nanospheres, metallic nanospheres, nanorods, carbon nanotubes, Si-nanotubes, nanowires, single-wall nanotubes, multi-wall nanotubes and combinations thereof.
7. A method of determining a dosage of a nanoparticle active agent comprising the steps of:
incubating a nanoparticle solution comprising one or more nanoparticles and one or more active agents with one or more cells;
isolating the one or more cells;
lysing the one or more cells to release a cell lysate;
separating the cell lysate electrophoretically on a gel;
digitizing the gel to form a gel image;
quantifying the nanoparticle intensity in the gel image; and
correlating the nanoparticle intensity to a concentration of the one or more active agents, wherein the concentration of the active agent in the one or more cells correlates to the dosage.
8. The method of claim 7 , wherein the one or more nanoparticles comprise fullerenes, quantum dots, inorganic nanospheres, metallic nanospheres, nanorods, carbon nanotubes, Si-nanotubes, nanowires, single-wall nanotubes, multi-wall nanotubes and combinations thereof.