Production of isomaltooligosaccharides and uses therefor
Described herein are methods for preparing isomaltooligosaccharides (“IMOs”) from a carbohydrate substrate and uses thereof. In the presence of a maltogenic enzyme and additional IMO precursors, an Aspergillus sp. invertase is capable of producing IMOs from a starch slurry. The ability of the invertase to function as a transglucosidase enzyme imparts a simultaneous mechanism for IMO saccharification.
1. A method for making an isomaltooligosaccharide composition comprising the steps of:
(a) contacting a starch slurry with a liquefaction enzyme at a temperature of about 100° C. to about 108° C. and pH of about 5-7 for 60-210 min; and
(b) contacting the product of step (a) with a maltogenic enzyme and an invertase at a temperature above 10° C. and below 85° C. and at a pH above 2 and below 10, wherein the maltogenic enzyme and the invertase are simultaneously contacted with the product of step (a) to produce isomaltooligosaccharides.
2. The method of claim 1 , wherein the slurry comprises 15% to 45% by weight of starch.
3. The method of claim 1 , wherein the invertase is at least 0.0009% by weight of all reactants of the step (b).
4. The method of claim 1 , wherein the invertase is less than 2% by weight of all reactants of the step (b).
5. The method of claim 1 , wherein the isomaltooligosaccharides are selected from the group consisting of isomaltose, branched glucose, DP3 glucose, DP3 glucose having alpha-1,4 and/or alpha-1,6 linkages, panose, isomaltotriose, isomaltotetraose, isomaltopentaose, isomaltohexaose, and isomaltoheptose, or any combination thereof.
6. The method of claim 5 , wherein at least 10% w/w of the produced isomaltooligosaccharides is panose.
7. The method of claim 1 , further comprising purifying the isomaltooligosaccharide composition.
8. The method of claim 1 , wherein the liquefaction enzyme is an alpha-amylase.
9. The method of claim 1 , wherein the maltogenic enzyme is a beta-amylase or a fungal alpha-amylase.
10. The method of claim 1 , wherein the invertase has transglucosidase activity.
11. The method of claim 1 , wherein the invertase is an Aspergillus invertase.
12. The method of claim 11 , wherein the Aspergillus invertase is an Aspergillus niger invertase or an Aspergillus oryzae invertase.
13. The method of claim 1 , wherein the slurry is formed from one or more starches and liquid.
14. The method of claim 1 , further comprising adding pullulanase to step (b).
15. The method of claim 1 , wherein the starch slurry comprises one or more substrates selected from the group consisting of corn starch, rice starch, wheat starch, tapioca, potato starch, sweet potato starch, sago starch, barley starch, heat/acid treated starch, pearl starch, waxy corn starch, sorghum starch, high amylose corn starch, liquid dextrose, and combinations thereof.
16. The method of claim 15 , wherein the starch slurry comprises corn starch.