Wall-associated kinase-like polypeptide mediates nutritional status perception and response
View Patent ↗The disclosure relates to methods for modulating plant growth and organogenesis using dominant-negative receptor-like kinases. The disclosure further provides a method for increasing plant yield relative to corresponding wild type plants comprising modulating the expression in a plant of a nucleic acid encoding a Wall-Associated Kinase-like 14 polypeptide or a homolog thereof, and selecting for plants having increased yield or growth on a nutrient deficient substrate.
1. A method comprising introducing and expressing in a plant a polynucleotide that is operably linked to a constitutive promoter, wherein the polynucleotide encodes a polypeptide having at least 95% identity to SEQ ID NO:2, and wherein expression or over expression of the polypeptide in the plant results in tolerance to nitrogen and/or sucrose deficient growth.
2. The method according to claim 1 , wherein the polynucleotide comprises a sequence that is at least 80% identical to SEQ ID NO:1.
3. The method according to claim 2 , wherein the polynucleotide is overexpressed in a plant.
4. The method according to claim 2 , wherein the polynucleotide comprises a sequence that is at least 95% identical to SEQ ID NO:1.
5. The method according to claim 4 , wherein the polynucleotide comprises the sequence of SEQ ID NO:1.
6. A transgenic plant produced by the method according to claim 1 or 2 .
7. A transgenic plant produced by the method of claim 1 or 2 , wherein the transgenic plant overexpresses the polynucleotide and wherein the transgenic plant has improved growth on a nutrient deficient media.
8. A method for the production of a transgenic plant having increased yield or growth relative to a corresponding wild type plant on a nutrient deficient media, which method comprises: (i) introducing and expressing in a plant or plant cell a polynucleotide that comprises a sequence that encodes a polypeptide having at least 95% identity to SEQ ID NO:2; and (ii) cultivating the plant cell under conditions for promoting plant growth and development.
9. A transgenic plant having increased tolerance to nitrogen and/or sucrose deficient conditions relative to a corresponding wild type plant, said increased tolerance resulting from a polynucleotide that encodes a polypeptide having at least 95% identity to SEQ ID NO:2 introduced into said plant.
10. The transgenic plant according to claim 9 , wherein said plant is a monocotyledonous plant, selected from the group consisting of sugar cane, rice, maize, wheat, barley, millet, rye, oats, triticale, and sorghum.
11. A transgenic plant part, or a transgenic plant cell, isolated from the transgenic plant of claim 6 .