Integrated analytical system and method
An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.
1. A system for measuring analytical reactions comprising:
a socket for holding an optode array chip comprising a two dimensional array of optode elements, each optode element comprising:
a nanoscale aperture within an aperture layer, the aperture forming a fluid receiving nanoscale well for receiving a fluid including fluorescent species;
above the aperture layer a fluidic layer in fluidic contact with the nanoscale well;
below the aperture layer a waveguide layer that provides illumination to the nanoscale well, the waveguide layer comprising an array of channel waveguides, each channel waveguide extending across a plurality of optode elements;
below the waveguide layer a transmission layer that transmits light emitted from the fluorescent species in the nanoscale well to a detector layer; and
below the transmission layer the detector layer comprising a detector which receives and detects the emitted light from the nanoscale well and transmitted through the transmission layer;
a fluidics delivery system for delivering fluid to the optode array elements; and
an illumination system that delivers illumination to waveguides of the optode array elements;
wherein the socket has electrical contacts that mate with electrical contacts on the chip, whereby electrical signals from the detector relating to detected emitted light are transmitted off of the chip through the electrical contacts.
2. The system of claim 1 wherein each optode element further comprises a lens or an optical tunnel that preferentially directs optical signals from the nanoscale well to the detector.
3. The system of claim 1 wherein the chip comprises from about 1000 to about one million optode elements.
4. The system of claim 1 wherein the detector layer comprises embedded circuits for processing output data from the detector.
5. The device of claim 4 wherein the embedded circuits filter background noise from the detector.
6. The system of claim 4 further comprising an amplifier for amplifying the output data.
7. The system of claim 1 further comprising an analog to digital converter.
8. The system of claim 1 wherein there is little or no free space between the aperture, waveguide and transmission layers.
9. The system of claim 1 wherein the detector layer can be reversibly separated from the portion of the array comprising the aperture layer.
10. The system of claim 1 further comprising a signal processing system downstream from the chip for processing and interpreting the data.
11. The system of claim 10 wherein the processing system includes a plurality of optional field programmable gate array (FPGA) blocks and application-specific integrated circuits (ASIC).
12. The system of claim 10 wherein the processing system includes a central processing unit (CPU) for processing data and controlling the system.
13. The system of claim 1 wherein the socket comprises a clamshell socket.
14. The system of claim 1 wherein the analytical reactions comprise nucleic acid sequencing.