Stabilized compositions of proteins having a free thiol moeity

1. A pharmaceutical composition comprising (i) an antibody or antibody fragment having a free thiol; (ii) a carbohydrate present in an amount sufficient to inhibit the aggregation of the antibody or antibody fragment; and (iii) O 2 gas, wherein the O 2 gas is less than about 10% of the gas in the composition, wherein the pH of the composition is less than 7.0, wherein the composition has less than 50% increase in the amount of antibody or antibody fragment aggregation as compared to the amount of antibody or antibody fragment aggregation that was in the composition prior to storage at a temperature of 2-8° C. for a period of up to 6 months, and wherein the aggregation is measured by size exclusive chromatography.

2. The pharmaceutical composition of claim 1 , further comprising an antioxidant, wherein the antioxidant and the carbohydrate are present in amounts sufficient to inhibit the aggregation of the antibody or antibody fragment.

3. The pharmaceutical composition of claim 1 , further comprising a surfactant.

4. The pharmaceutical composition of claim 1 , wherein the pH of the composition is between about 4.5 and about 6.5.

5. The pharmaceutical composition of claim 1 , wherein the inhibition of antibody or antibody fragment aggregation is at least 5-80% greater, under pre-selected conditions, than the inhibition of antibody or antibody fragment aggregation in a composition which differs by lacking the carbohydrate.

6. The pharmaceutical composition of claim 1 , wherein the carbohydrate is present in an amount sufficient to inhibit the degradation of the antibody or antibody fragment, wherein the composition has less than 50% increase in the amount of antibody or antibody fragment degradation as compared to the amount of antibody or antibody fragment degradation that was in the composition prior to storage at a temperature of 2-8° C. for a period of up to 6 months, and wherein the degradation is measured by reverse phase HPLC.

7. The pharmaceutical composition of claim 1 , wherein the carbohydrate is present in an amount sufficient to inhibit the reaction of a free thiol on a first molecule of the antibody or antibody fragment with a free thiol on a second molecule of the antibody or antibody fragment to form an aggregate.

8. The pharmaceutical composition of claim 1 , wherein the carbohydrate is present in an amount sufficient to inhibit the formation of an aggregate formed by the reaction of a free thiol on a first molecule of the antibody or antibody fragment with a free thiol on a second molecule of the antibody or antibody fragment by at least 5-80%, under pre-selected conditions, as compared to the same composition lacking the carbohydrate.

9. The pharmaceutical composition of claim 1 , wherein the storage occurs in a gas tight container.

10. The pharmaceutical composition of claim 9 , wherein the storage occurs in darkness.

11. The pharmaceutical composition of claim 1 , wherein the inhibition of antibody or antibody fragment aggregation is comparable to that of a lyophilized composition comprising sucrose, 0.01% polysorbate-20, pH 6.0, 50 mM Citrate.

12. The pharmaceutical composition of claim 1 , comprising about 1-40% carbohydrate.

13. The pharmaceutical composition of claim 1 , wherein the carbohydrate is sucrose or trehalose.

14. The pharmaceutical composition of claim 1 , wherein the composition is a liquid.

15. The pharmaceutical composition of claim 1 , wherein the composition is made by a method comprising physical removal of O 2 from the composition.

16. The pharmaceutical composition of claim 1 , wherein the antibody or antibody fragment having a free thiol has two, three, or more free thiol groups and has zero, two, four, or more thiol groups which form sulfhydryl bridges, per active unit of protein.

17. A gas tight container comprising the pharmaceutical composition of claim 1 and a headspace, wherein the headspace is at least 90% (vol/vol) an inert gas.

18. The gas tight container of claim 17 , wherein the container is prefilled syringe, a vial, or ampoule.

19. The gas tight container of claim 18 , wherein the prefilled syringe is a needleless syringe.

20. A method of packaging the pharmaceutical composition of claim 1 , the method comprising contacting the antibody or antibody fragment having a free thiol with an inert gas to reduce the amount of a reactive species, and introducing the antibody or antibody fragment and the inert gas into a gas tight container.

21. The method of claim 20 , wherein the inert gas is N 2 or Ar and the reactive species is O 2 .

22. In a method of treating a patient with a pharmaceutical composition comprising an antibody, the improvement comprising administering the pharmaceutical composition of claim 1 to the patient.

23. The method of claim 22 , wherein the administration is by IV infusion or subcutaneous administration.

24. The pharmaceutical composition of claim 1 , wherein the antibody or antibody fragment is a human antibody or antibody fragment.

25. The pharmaceutical composition of claim 1 , wherein the antibody or antibody fragment is an IgA, IgG, or IgM antibody or antibody fragment.

26. The pharmaceutical composition of claim 1 , wherein the antibody fragment is an Fab fragment, an F(ab′) 2 fragment, or a single-chain Fv fragment (scFv).

27. The gas tight container of claim 17 , wherein the antibody or antibody fragment is a human antibody or antibody fragment.

28. The gas tight container of claim 17 , wherein the antibody or antibody fragment is an IgA, IgG, or IgM antibody or antibody fragment.

29. The gas tight container of claim 17 , wherein the antibody fragment is an Fab fragment, an F(ab′) 2 fragment, or a single-chain Fv fragment (scFv).