Methods for determining the risk of prenatal complications
View Patent ↗The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers.
1. A method for determining the risk of a chromosomal abnormality in a fetus, comprising:
determining the amount of placental growth factor (PlGF), pregnancy-associated plasma protein A (PAPP-A), and free human chorionic gonadotropin (free beta hCG) in one or more blood samples taken from a pregnant individual; and determining the risk of the chromosomal abnormality in the fetus using the measured amounts of PlGF, PAPP-A, and free beta hCG.
2. The method of claim 1 , wherein the chromosomal abnormality is selected from the group consisting of trisomy 21, trisomy 18, trisomy 13, Turner syndrome, and triploidy.
3. The method of claim 1 , further comprising determining one or more ultrasound markers of the fetus and determining the risk of the chromosomal abnormality in the fetus using the amounts of PlGF, PAPP-A, free beta hCG, and the one or more ultrasound marker of the fetus.
4. The method of claim 3 , wherein the ultrasound marker is nuchal translucency.
5. The method of claim 1 , further comprising determining the amount of at least one biochemical marker selected from placental protein 13 (PP13) and metalloprotease 12 (ADAM12), and determining the risk of the chromosomal abnormality in the fetus using the amounts of PlGF, PAPP-A, free beta hCG, and the at least one biochemical marker.
6. The method of claim 1 , wherein the one or more blood samples are taken from the pregnant individual in the first trimester of pregnancy.
7. The method of claim 1 , wherein the one or more blood samples are taken from the pregnant individual within weeks 10 to 19 of pregnancy.
8. The method of claim 1 , wherein the one or more blood samples are taken from the pregnant individual within weeks 11 to 13 of pregnancy.
9. The method of claim 1 , wherein the determining comprises calculating a final risk based on the prior risk of developing the chromosomal abnormality and a set of likelihood ratios based on the amounts of PlGF, PAPP-A, and free beta hCG.
10. The method of claim 9 , wherein multivariate Gaussian analysis is performed to determine the likelihood ratios.
11. The method of claim 9 , further comprising using likelihood ratios for one or more maternal history parameters.
12. A medical profile for a pregnant individual, comprising information for determining risk of a chromosomal abnormality in a fetus, wherein the information comprises the amounts of PlGF, PAPP-A, and free beta hCG in one or more blood samples from the pregnant individual, and wherein the medical profile is stored on a computer-readable medium.
13. The medical profile of claim 12 , wherein the medical profile further comprises additional information for determining the risk of developing pre-eclampsia, wherein the additional information comprises the blood pressure of the pregnant individual.
14. An apparatus for determining risk of a chromosomal abnormality in a fetus, the apparatus comprising:
a data input means for inputting the amounts of PlGF, PAPP-A, and free beta hCG in one or more blood samples obtained from a pregnant individual; and
a calculation means for determining the risk of a chromosomal abnormality in a fetus using the amounts of the PlGF, PAPP-A, and free beta hCG.
15. The apparatus of claim 14 , further comprising a data input means for inputting at least one of the amounts of ADAM12 and PP13 in one or more blood samples obtained from the pregnant individual; and determining the risk of a chromosomal abnormality in a fetus using the amounts of at least one of ADAM12 and PP13, and the amounts of PlGF, PAPP-A, and free beta hCG.
16. The apparatus of claim 14 , wherein the apparatus further determines risk of developing pre-eclampsia, the apparatus further comprising:
a data input means for inputting a blood pressure of the pregnant individual; and
a calculation means for determining the risk of pre-eclampsia using the input amounts of one or more of PlGF and PAPP-A, and the blood pressure.