Method of inducing high activity of human adipose stem cell and medium therefor
The present invention relates to a method of inducing high activity of human adipose stem cells, highly active stem cells induced by the method, cell therapeutic agents including the highly active stem cells, and a medium for inducing high activity of human adipose stem cells. The method of the present invention enables a long-term culture of human adipose stem cells while maintaining high activity, production yield and differentiation potency of the stem cells through in vitro culture, even in case culture conditions are not appropriate for mature human adipocytes, security of adipocytes is not guaranteed, or adipocytes are diseased.
1. A method for inducing high activity of human mesenchymal stem cells, the method comprising culturing human mesenchymal stem cells in a medium containing a compound represented by the following Formula 1 at a concentration of 1-100 μM:
wherein R 1 , R 2 and R 3 are H, C 1 -C 3 alkyl or C 1 -C 4 acyl.
2. The method according to claim 1 , wherein the medium further comprises a serum.
3. The method according to claim 2 , wherein the serum is selected from the group consisting of fetal bovine serum (FBS), bovine calf serum (BCS), horse serum, and human serum.
4. The method according to claim 1 , wherein the medium is selected from the group consisting of α-MEM, Eagle's MEM, Iscove's MEM, 199 medium, CMRL 1066, RPMI 1640, F12, F10, DMEM, Waymouth's MB752/1 and McCoy's 5A.
5. The method according to claim 2 , wherein the serum is included in the medium in an amount of 4-30 wt %.
6. The method according to claim 1 , wherein the mesenchymal stem cells are adipose stem cells.