HYR1 as a target for active and passive immunization against
The invention features HYR1 as a vaccine target and as a prophylactic strategy for combating disseminated candidiasis.
1. An immunogenic composition comprising an isolated polypeptide optionally fused to a heterologous fusion sequence and a pharmaceutically acceptable carrier, wherein the amino acid sequence of said polypeptide consists of the amino acid sequence of SEQ ID NO: 2.
2. The immunogenic composition of claim 1 , further comprising an adjuvant.
3. The immunogenic composition of claim 1 , wherein the polypeptide is fused to the heterologous fusion sequence.
4. The immunogenic composition of claim 3 , wherein the heterologous fusion sequence is a heterologous leader sequence, a tag, or a linker sequence.
5. The immunogenic composition of claim 4 , wherein the tag is a histidine tag.
6. The immunogenic composition of claim 4 , wherein the polypeptide is obtained from a transformed cell.
7. The immunogenic composition of claim 6 , wherein the transformed cell is a transformed Saccharomyces cerevisiae cell.
8. The immunogenic composition of claim 4 , wherein the composition is a vaccine composition for immunization against Candida albicans and the polypeptide is purified.
9. A method of immunizing a mammalian host against Candida albicans comprising administering to said host an immunogenically effective amount of the vaccine composition of claim 8 .
10. The method of claim 9 , wherein the vaccine composition further comprises an adjuvant.