Enzyme feed additive and animal feed including it
The use is provided composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma . Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase.
1. A method of manufacturing an endoglucanase-containing animal feed additive, comprising expressing one or more active endoglucanases in a cell culture, recovering said one or more active endoglucanases, and formulating said recovered one or more active endoglucanases into said feed additive, wherein said feed additive is free of any cellobiohydrolase, and wherein a first animal feed comprising said active endoglucanase-containing animal feed additive, when fed to an animal, results in a first feed conversion ratio that is at least 3.5% lower than a second feed conversion ratio resulting from the consumption of a second animal feed that lacks said endoglucanase-containing feed additive.
2. The method according to claim 1 , wherein at least one of said endoglucanases is a fungal endoglucanase.
3. The method according to claim 1 , wherein at least one of said endoglucanases is selected from the group consisting of EGI, EGII, EGIII, and a catalytic core fragment of any thereof.
4. The method according to claim 2 , wherein said endoglucanase is from Trichoderma.
5. The method according to claim 2 , wherein said endoglucanase is from T. longibrachiatum, T. reesei or T viride.
6. The method according to claim 1 , wherein at least one of said endoglucanases lacks a cellulose binding domain.
7. The method according to claim 1 , wherein said first feed conversion ratio is at least 7% lower than said second feed conversion ratio.
8. A method of manufacturing an endoglucanase-containing animal feed additive, comprising expressing one or more active endoglucanases in a cell culture, recovering said one or more active endoglucanases, and formulating said recovered one or more active endoglucanases into said feed additive, wherein said additive is free of any cellobiohydrolase, and wherein a first animal feed comprising said active endoglucanase-containing animal feed additive, when fed to an animal, results in a first gastrointestinal viscosity that is at least 5.2% lower than a second gastrointestinal viscosity resulting from the consumption of a second animal feed that lacks said endoglucanase-containing feed additive.
9. The method according to claim 8 , wherein at least one of said endoglucanases is a fungal endoglucanase.
10. The method according to claim 8 , wherein at least one of said endoglucanases is selected from the group consisting of EGI, EGII, EGIII, and a catalytic core fragment of any thereof.
11. The method according to claim 9 , wherein said endoglucanase is from Trichoderma.
12. The method according to claim 9 , wherein said endoglucanase is from T. longibrachiatum, T. reesei or T. viride.
13. The method according to claim 8 , wherein at least one of said endoglucanases lacks a cellulose binding domain.
14. The method according to claim 8 , wherein said first gastrointestinal viscosity is at least 11% lower than said second gastrointestinal viscosity.