JAB1 as a prognostic marker and a therapeutic target for human cancer
View Patent ↗Methods of diagnosing and prognosticating the development of human cancers, such as breast cancer, colon cancer, and pancreatic cancer, are provided. The diagnostic and prognostic methods include the detection and/or quantifying of the amount of expression of JAB1 in human cells, particularly in relation to the amount of p27 or c-Jun. In addition, methods for reducing the expression of JAB1 protein in cells and inhibiting its interaction with p27 or c-Jun, for example, are provided.
1. A method of determining a prognosis for cancer in an individual, the method comprising assessing a JAB1 protein labeling index in a sample of cancer cells, or cells suspected of being cancerous, from the individual, wherein a sample having a JAB1 labeling index of greater than 50% is indicative of a poor prognosis, wherein the cancer is ovarian cancer, breast cancer, non-Hodgkin's lymphoma, colon cancer, prostate cancer, pancreatic cancer, liver cancer, colorectal or lung cancer.
2. The method of claim 1 , wherein the cancer is breast cancer, non-Hodgkin's lymphoma, liver cancer, colon cancer or colorectal cancer.
3. The method of claim 2 , wherein the cancer is breast cancer.
4. The method of claim 1 , wherein a JAB1 labeling index is defined as that level of JAB1 expression achieved when 50% or greater of cells in the sample are detectable by immunohistochemical testing using a JAB1 antibody.
5. The method of claim 1 , further comprising assessing a p27 labeling index in the sample, wherein a sample having a p27 labeling index of lower than 50% is indicative of a poor prognosis.
6. The method of claim 1 , wherein the JAB1 labeling index is assessed by immunohistochemistry, ELISA or FISH.
7. The method of claim 5 , wherein the p27 labeling index is assessed by immunohistochemistry, ELISA or FISH.
8. The method of claim 1 , wherein the cancer is non-Hodgkin's lymphoma.
9. The method of claim 1 , wherein the cancer is colon cancer.
10. The method of claim 1 , wherein the cancer is prostate cancer.
11. The method of claim 1 , wherein the cancer is pancreatic cancer.
12. The method of claim 1 , wherein the cancer is liver cancer.
13. The method of claim 1 , wherein the cancer is colorectal cancer.
14. The method of claim 1 , wherein the cancer is lung cancer.
15. The method of claim 1 , wherein the cancer is ovarian cancer.