Multi-way sorter system and method
Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.
1. A carrier for receiving cells sorted from a cell sorter system, the carrier comprising:
a plurality of hollow deflection tubes, wherein each deflection tube has a first opening at a first end and a second opening at a second end, wherein the first opening of each deflection tube is aligned with the first opening of an adjacent deflection tube such that the first openings are all collinear, and wherein the second openings of the plurality of deflection tubes are staggered with respect to one another;
a plurality of collection tubes corresponding to the plurality of deflection tubes, wherein each collection tube includes an opening positioned to receive a cell passed through and exiting a corresponding deflection tube; and
a current detection circuit coupled to at least one deflection tube.
2. The carrier of claim 1 , wherein each deflection tube is electrically insulated.
3. The carrier of claim 1 , wherein each collection tube is electrically insulated.
4. The carrier of claim 1 , further comprising: a current detection circuit coupled to each deflection tube.
5. The carrier of claim 1 , wherein the current detection circuit further comprises: a current-to-voltage converter to detect the presence of a charged drop entering the deflection tube.
6. The carrier of claim 1 , wherein the current detection circuit further comprises: an integrator to detect a number of drops per-unit-time entering the deflection tube.
7. The carrier of claim 1 , wherein each deflection tube is formed of a metallic material.
8. The carrier of claim 1 , wherein each collection tube is formed of a plastic material.
9. The carrier of claim 1 , wherein the carrier includes ten or more deflection tubes and ten or more corresponding collection tubes.
10. A system comprising a cell sorter and the carrier of claim 1 wherein the carrier is configured to receive cells from the cell sorter.
11. The cell sorter system of claim 10 , further comprising: a computer processor configured to identify when cells passed through a respective deflection tube are not in line with expectations.
12. The cell sorter system of claim 11 , further comprising: a motor to move the carrier to a deactivated position.
13. A method for configuring a carrier for receiving cells sorted by a cell sorter system, the method comprising: aligning a plurality of hollow deflection tubes such that a first open end of each deflection tube is positioned collinearly with a first open end of an adjacent deflection tube, and wherein a second open end of each deflection tube is positioned staggered with respect to a second open end of an adjacent deflection tube; positioning a plurality of corresponding collection tubes such that an opening for each collection tube is positioned to receive a cell passed through and exiting a corresponding deflection tube and coupling a current detection circuit to at least one deflection tube.
14. The method of claim 13 , wherein each deflection tube is electrically insulated.
15. The method of claim 13 , wherein each collection tube is electrically insulated.
16. The method of claim 13 , further comprising: coupling a current detection circuit to each deflection tube.
17. The method of claim 13 , wherein the current detection circuit further comprises: a current-to-voltage converter to detect the presence of a charged drop entering the deflection tube.
18. The method of claim 13 , wherein the current detection circuit further comprises: an integrator to detect a number of drops per-unit-time entering the deflection tube.
19. The method of claim 13 , wherein each deflection tube is formed of a metallic material.
20. The method of claim 13 , wherein each collection tube is formed of a plastic material.
21. The method of claim 13 , wherein the carrier includes ten or more deflection tubes and ten or more corresponding collection tubes.