Influenza hemagglutinin and neuraminidase variants
Polypeptides, polynucleotides, methods, compositions, and vaccines comprising (avian pandemic) influenza hemagglutinin and neuraminidase variants are provided.
1. A reassortant influenza virus, wherein said virus comprises 6 internal genome segments from A/Ann Arbor/6/60 and a genome segment encoding an immunogenic influenza surface antigen comprising the amino acid sequence of SEQ ID NO: 13.
2. An immunogenic composition comprising an immunologically effective amount of the reassortant influenza virus of claim 1 .
3. A method for stimulating the immune system of a subject to produce a protective immune response against influenza virus, the method comprising administering to the subject an immunologically effective amount of the reassortant influenza virus of claim 1 in a physiologically effective carrier.
4. A method of prophylactic or therapeutic treatment of a viral infection in a subject, the method comprising: administering to the subject, the virus of claim 1 in an amount effective to produce an immunogenic response against the viral infection.
5. The method of claim 4 , wherein said virus is killed or inactivated.
6. The method of claim 3 , wherein the subject is a human.
7. A live attenuated influenza vaccine comprising the immunogenic composition of claim 2 .
8. A split virus or killed virus vaccine comprising the immunogenic composition of claim 2 .
9. A method or producing influenza viruses in cell culture, the method comprising:
i) introducing into a population of host cells a plurality of vectors comprising nucleic acid sequences corresponding to-at least 6 internal genome segments from A/Ann Arbor/6/60 and at least one genome segment encoding an immunogenic influenza surface antigen comprising an amino acid sequence of: SEQ ID NO: 13;
ii) culturing the population of host cells at a temperature less than or equal to 35° C.; and,
iii) recovering a plurality of influenza viruses.
10. An immunogenic composition comprising an immunologically effective amount of an influenza virus produced by a method comprising:
i) introducing into a population of host cells a plurality of vectors comprising nucleic acid sequences corresponding to-at least 6 internal genome segments from A/Ann Arbor/6/60 and a genome segment encoding an immunogenic influenza surface antigen comprising the amino acid sequence of SEQ ID NO: 13;
ii) culturing the population of host cells at a temperature less than or equal to 35° C.; and;
iii) recovering a plurality of influenza viruses.
11. A method for stimulating the immune system of an individual to produce a protective immune response against influenza virus, the method comprising administering to the individual an immunologically effective amount of the influenza virus produced by the method of claim 9 in a physiologically effective carrier.
12. A method for stimulating the immune system of an individual to produce a protective immune response against influenza virus, the method comprising administering to the individual the immunogenic composition of claim 11 .