IP Library › Granted Patent US 8,765,922
Granted Patent B2
US 8,765,922 · App. 13/277,082 · Granted Jul 1, 2014

Device and method for separation of Neél- and brown-magnetic particles

Inventors: Jürgen Schulz (Jesteburg, DE); Daniel Michalek (Engelskirchen, DE); Dirk Merkel (Solingen, DE); Stefan Miltenyi (Bergisch-Gladbach, DE)
Assignee: Miltenyi Biotec GmbH
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Quick Facts
Patent No.
US 8,765,922
App. No.
13/277,082
Granted
Jul 1, 2014
Kind
B2
Abstract

A method and associated device for enrichment of Neél-magnetic particles from a dispersion of Brown-magnetic particles and Neél-magnetic particles. The device and method use ferromagnetic separation particles having a mean diameter of 100 to 250 μm located in a alternating magnetic field. The ferromagnetic separation particles have a magnetically and chemically inert coating.

Claims (16)

1. A device for enrichment of Neél-magnetic particles from a dispersion of Brown-magnetic particles and Neél-magnetic particles, comprising:

a column holding ferromagnetic separation particles having a mean diameter of 100 to 250 μm; and

an electromagnetic element which generates an alternating magnetic field, wherein the column is located in the alternating magnetic field; and wherein the separation particles have a magnetically and chemically inert coating.

2. A device according to claim 1 , wherein the inert coating has a thickness in the range of 1 to 25 μm.

3. A device according to claim 1 , wherein the inert coating includes an organic lacquer or an inorganic material.

4. A device according claim 1 , wherein the electromagnetic element generates an alternating magnetic field which has a frequency in the range of 40 to 200 kHz.

5. A method for enrichment of Neél-magnetic particles from a dispersion of Brown-magnetic particles and Neél-magnetic particles, comprising the acts of:

directing the dispersion at least once over ferromagnetic separation particles having a mean diameter in the range of 100 to 250 μm; and

applying an alternating magnetic field to the dispersion, thereby immobilizing the Neél-magnetic particles at the separation particles; wherein the separation particles have a magnetically and chemically inert coating.

6. A method according to claim 5 , wherein the inert coating has a thickness in the range of 1 to 25 μm.

7. A method according to claim 5 , wherein the inert coating includes an organic lacquer or an inorganic material.

8. A method to claim 5 , wherein the act of directing is repeated at least once over the separation particles and through the alternating magnetic field.

9. A method according to claim 5 , further comprising the acts of:

removing the separation particles with the Neél-magnetic particles immobilized thereon from the alternating magnetic field; and

eluting the Neél-magnetic particles from the separation particles thereby to obtain a dispersion enriched in Neél-magnetic particles.

10. A method according to claim 9 , further comprising the act of directing the enriched dispersion through a static magnetic field, thereby immobilizing and separating the Neél-magnetic particles.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 30, 2012
From: SCHULZ, JURGEN; MICHALEK, DANIEL; MERKEL, DIRK; MILTENYI, STEFAN
To: MILTENYI BIOTEC GMBH
Reel/Frame 027620/0250 →
Priority Claims (1)
DE 10 2010 042 723 · Oct 20, 2010 · national
Continuity (1)
Related Publication 20120273395A1 · Nov 1, 2012