Artificial cell constructs for cellular manipulation
The present invention contemplates induction of immunological tolerance thereby providing permanent allograft acceptance. This method obviates the need for a lifelong regimen of immunosuppressive agents which can increase the risk of infection, autoimmunity, and cancer. Immunological tolerance is thought to be mediated by regulatory T lymphocytes (T reg cells) with immunosuppressive capabilities. A therapeutically relevant platform comprising artificial constructs are contemplated comprising numerous soluble and surface bound T reg cell stimulating factors that may induce tolerance following allograft transplantation. Such artificial constructs, being the size of a cell, have surface bound monoclonal antibodies specific to regulatory T-cell surface moieties and encapsulated soluble regulatory T-cell modulating factors.
1. A method, comprising:
a) providing,
i) a patient, wherein said patient comprises a Treg cell population and at least one inflammation site;
ii) an artificial particle comprising a soluble Treg cell chemoattractant factor consisting of CCL22; and
b) administering said artificial particle to said patient at said at least one inflammation site under conditions such that said Treg cell population migrates towards said at least one inflammation site such that said Treg cell population is enriched.
2. The method of claim 1 , wherein said patient has under gone a procedure selected from the group consisting of periodontal surgery, scaling and root planing.
3. The method of claim 1 , wherein said at least one site of inflammation comprises a tissue injury.
4. The method of claim 1 , wherein said at least one site of inflammation comprises a periodontal disease.
5. The method of claim 1 , wherein said artificial particle is porous.
6. The method of claim 1 , wherein said artificial particle is biodegradable.
7. The method of claim 1 , wherein said at least one soluble Treg cell factor undergoes a controlled release.
8. The method of claim 7 , wherein said controlled release is a short term release.
9. The method of claim 7 , wherein said controlled release is a long term release.
10. The method of claim 1 , wherein said at least one inflammation site comprises a cancer disease.