One dose vaccination against
The present invention provides a one phase, aqueous vaccine composition for immunizing an animal against infection by Mycoplasma hyopneumoniae , comprising: an immunizing amount of a Mycoplasma hyopneumoniae bacterin, an acrylic acid polymer in the concentration range between 0.8 and 1.2 mg/ml, and a pharmaceutically acceptable carrier, and substantially no oil. It is especially useful for immunizing a pig against infection by Mycoplasma hyopneumoniae for at least 20 weeks after a single administration, which effective immunity is reached within 4 weeks after vaccination.
1. A method of preparing a one phase aqueous composition comprising a Mycoplasma hyopneumoniae bacterin, an acrylic acid polymer in a concentration range between 0.8 and 1.2 mg/ml, a pharmaceutically acceptable carrier, and substantially no oil;
comprising:
a. cultivating a M. hyopneumoniae whole cell culture such that the Mycoplasma hyopneumoniae bacterin has an antigen amount of at least 3 log 10 per ml;
b. inactivating the M. hyopneumoniae whole cell culture;
c. isolating an immunizing amount of a Mycoplasma hyopneumoniae bacterin;
d. admixing an acrylic acid polymer in the concentration range between 0.8 and 1.2 mg/ml; and
e. admixing a pharmaceutically acceptable carrier.
2. The method of claim 1 , wherein the M. hyopneumoniae whole cell culture is inactivated by a method selected from the group consisting of heat denaturation, shear force, and a chemical agent.
3. The method of claim 2 , wherein the chemical agent is selected from the group consisting of binary ethyleneimine (BEI) and formalin.
4. The method of claim 1 , wherein the one phase, aqueous composition consists essentially of no other ingredients.
5. The method of claim 1 , wherein said one phase, aqueous composition consists essentially of substantially pure formalin-inactivated whole cell Mycoplasma hyopneumoniae J (ATCC 25934) bacterin having an antigen amount between 5 log 10 and 8 log 10 per ml before inactivation; about 1 mg/ml carbomer; and up to 1 ml 0.85% (w/v) sodium chloride aqueous solution.