Process for preparing unaggregated antibody Fc domains
Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.
1. A method for producing a solution of purified unaggregated Fc peptide chains comprising the steps of:
a) providing an aqueous sample comprising unaggregated Fc peptide chains and aggregated Fc peptide chains;
b) contacting the sample with a population of protein A peptide chains bound to an insoluble material and capable of binding greater than 70% of the total mass of unaggregated Fc peptide chains and aggregated Fc peptide chains in the sample;
c) contacting the population of protein A peptide chains with an aqueous solution having a salt concentration consisting of tris(hydroxymethyl)aminomethane acetate of 41 mM to 97 mM and a pH of 3.42 to 3.74; and
d) removing the aqueous solution from contact with the population of protein A peptide chains to produce a solution of purified unaggregated Fc peptide chains comprising less then 5% aggregated Fc peptide chains;
wherein the total percent yield of Fc peptide chains is greater than 70%.
2. The method of claim 1 wherein the population of protein A peptide chains bound to an insoluble material has a height equivalent to a theoretical plate value of 0.005 cm to 0.05 cm.
3. The method of claim 1 wherein the unaggregated Fc peptide chain is a mimetibody.
4. A method for producing a solution of purified unaggregated Fc peptide chains comprising the steps of:
a) providing an aqueous sample comprising unaggregated Fc peptide chains and aggregated Fc peptide chains;
b) contacting the sample with a population of protein A peptide chains bound to an insoluble material and capable of binding greater than 70% of the total mass of unaggregated Fc peptide chains and aggregated Fc peptide chains in the sample;
c) contacting the population of protein A peptide chains with an aqueous solution having a salt concentration consisting of tris(hydroxymethyl)aminomethane acetate of 55 mM to 85 mM and a pH of 3.50 to 3.66; and
d) removing the aqueous solution from contact with the population of protein A peptide chains to produce a solution of purified unaggregated Fc peptide chains comprising less then 5% aggregated Fc peptide chains;
wherein the total percent yield of Fc peptide chains is greater than 70%.
5. The method of claim 4 wherein the population of protein A peptide chains bound to an insoluble material has a height equivalent to a theoretical plate value of 0.005 cm to 0.05 cm.
6. The method of claim 4 wherein the unaggregated Fc peptide chain is a mimetibody.
7. A method for producing a solution of purified unaggregated mimetibody Fc peptide chains comprising the steps of:
a) providing an aqueous sample comprising unaggregated mimetibody Fc peptide chains and aggregated mimetibody Fc peptide chains;
b) contacting the sample with a population of protein A peptide chains bound to an insoluble material and capable of binding greater than 70% of the total mass of unaggregated mimetibody Fc peptide chains and aggregated mimetibody Fc peptide chains in the sample;
c) contacting the population of protein A peptide chains with an aqueous solution having a salt concentration consisting of tris (hydroxymethyl) aminomethane acetate of 55 mM to 85 mM and a pH of 3.50 to 3.66; and
d) removing the aqueous solution from contact with the population of protein A peptide chains to produce a solution of purified unaggregated mimetibody Fc peptide chains comprising less then 5% aggregated mimetibody Fc peptide chains;
wherein the total percent yield of mimetibody Fc peptide chains is greater than 70%.
8. The method of claim 7 wherein the population of protein A peptide chains bound to an insoluble material has a height equivalent to a theoretical plate value of 0.005 cm to 0.05 cm.