Monoclonal antibodies against HPV proteins
Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. Various monoclonal antibodies recognizing specific epitope for specific HPV protein or HPV type, common epitope for various HPV proteins or HPV types are obtained. These obtained monoclonal antibodies are useful tools in early clinical detection of HPV infection and general detection of HPV related diseases, specific detection of invasive cervical cancer, detection of other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression.
1. A monoclonal antibody that specifically binds to two or more native HPV proteins from different HPV types, wherein said two or more native HPV proteins are native E7 proteins from different HPV types or native E6 proteins from different HPV types, and wherein said monoclonal antibody binds in situ to said proteins in a clinical sample.
2. The monoclonal antibody of claim 1 , wherein the monoclonal antibody specifically binds to a native E7 protein from HPV 16 and a native E7 protein from HPV 18.
3. The monoclonal antibody of claim 1 , wherein the monoclonal antibody specifically binds to a native E6 protein from HPV 16 and a native E6 protein from HPV 18.
4. The monoclonal antibody of claim 1 , wherein the different HPV types are selected from the group consisting of high risk HPV types, low risk HPV types, HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, and HPV-68, HPV-6, HPV-11, HPV-42, HPV-43, HPV-44, HPV-53, HPV-54, HPV-55, and HPV-56.
5. A monoclonal antibody that specifically binds in situ in a clinical sample to two or more native E7 proteins from different HPV types.
6. The monoclonal antibody of claim 5 , wherein the monoclonal antibody specifically binds to a native E7 protein from HPV 16 and a native E7 protein from HPV 18.
7. A monoclonal antibody that specifically binds in situ in a clinical sample to a native HPV E7 protein and a native HPV E6 protein, wherein the native HPV E7 protein and the native HPV E6 protein are from the same HPV type.
8. The monoclonal antibody of claim 7 , wherein said native E7 protein and said native E6 protein are from HPV 16.
9. The monoclonal antibody of claim 7 , wherein said native E7 protein and said native E6 protein are from HPV 18.
10. The monoclonal antibody of claim 7 , wherein the native HPV E7 proteins and the native HPV E6 proteins are from the same HPV type selected from the group consisting of high risk HPV types, low risk HPV types, HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, and HPV-68, HPV-6, HPV-11, HPV-42, HPV-43, HPV-44, HPV-53, HPV-54, HPV-55, and HPV-56.