Patent
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
1. An isolated nucleic acid molecule encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein said nucleic acid molecule is operably linked to a heterologous promoter operable in a plant.
2. A DNA construct comprising a nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, or a full-length complement thereof; and
b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2; and
a heteroloqous promoter, operably linked to the nucleic acid molecule.
3. A host cell that contains the DNA construct of claim 2 .
4. The host cell of claim 3 that is a bacterial cell.
5. The host cell of claim 3 that is a plant cell.
6. A transgenic plant comprising the host cell of claim 5 .
7. The transgenic plant of claim 6 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugar beet, sugarcane, tobacco, barley, and oilseed rape.
8. Transformed seed of the plant of claim 7 , wherein the seed comprise the DNA construct.
9. A method for producing a polypeptide with pesticidal activity, comprising culturing the host cell of claim 3 under conditions in which a nucleic acid molecule encoding the polypeptide is expressed, said polypeptide being selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2; and
b) a polypeptide that is encoded by the nucleotide sequence of SEQ ID NO:1.
10. A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, and
b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
11. The plant of claim 10 , wherein said plant is a plant cell.
12. A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1; and
b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
13. The method of claim 12 , wherein said plant produces a pesticidal polypeptide having pesticidal activity against an insect pest.