Materials for magnetizing cells and magnetic manipulation
A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
1. A composition for magnetizing cells, said composition comprising:
a) a negatively charged nanoparticle;
b) a positively charged nanoparticle; and
c) a support molecule,
wherein one of said negatively charged nanoparticle or positively charged nanoparticle is a magnetically responsive element or compound, and wherein said support molecule holds said negatively charged nanoparticle and said positively charged nanoparticle in an intimate admixture forming a fibrous mat-like structure.
2. The composition of claim 1 , wherein said negatively charged nanoparticle is a gold nanoparticle.
3. The composition of claim 1 , wherein said positively charged nanoparticle is an iron oxide nanoparticle.
4. The composition of claim 1 , wherein the support molecule comprises peptides, polysaccharides, nucleic acids, polymers or combinations thereof.
5. The composition of claim 1 , wherein the support molecule comprises poly-lysine, fibronectin, collagen, laminin, BSA, hyaluronan, glycosaminoglycan, non-sulfated glycosaminoglycan, gelatin, nucleic acid, extracellular matrix proteins, cell extract, antibody or mixtures or derivatives thereof.
6. The composition of claim 1 , wherein the support molecule comprises polylysine.
7. The composition of claim 1 , wherein said cells are obtained from animal tissue or fluids, and said support molecule is provided by said tissue or fluids.
8. The composition of claim 1 ,
a) wherein the support molecule comprises poly-lysine,
b) wherein said negatively charged nanoparticle is a gold nanoparticle, and
c) wherein said positively charged nanoparticle is an iron oxide nanoparticle.
9. A composition comprising magnetized cells that are made by incubation with composition of claim 8 .
10. The composition of claim 9 , wherein said cells are frozen.
11. A method of moving cells, comprising contacting cells with the composition of claim 1 and incubating for 1-12 hours until the cells become magnetized, and subjecting said magnetized cells to a magnetic field sufficient to move said cells.
12. The method of claim 11 , wherein said magnetic field is asymmetric.
13. The method of claim 11 , wherein said cells are in suspension or adhered.
14. The method of claim 11 , further comprising washing said cells to remove said composition before subjecting said magnetized cells to a magnetic field sufficient to move said cells.
15. A method of delivery a target molecule to cells for 3D culturing, comprising contacting a suspension of cells with the composition of claim 1 plus a target molecule, incubating for 1-12 hours until the cells become magnetized and said target has been delivered to said cell, and applying a magnetic field to said magnetized cells to 3D culture said magnetized cells.
16. A composition comprising magnetized cells that are made by incubation with composition of claim 1 .
17. The composition of claim 16 , wherein said cells are frozen.