Method for identifying, expanding and removing adult stem cells and cancer stem cells
Described are the use of stem cell markers for the isolation of stem cells and the use of the obtained stem cells in, for example, research or treatment, such as for the preparation of a medicament for the treatment of damaged or diseased tissue. In one aspect, provided is a method for obtaining or isolating stem cells that comprises preparing a cell suspension from a tissue or organ sample, contacting the cell suspension with an Lgr 6 or 5 binding compound, identifying the cells bound to the binding compound, and isolating the stem cells from the binding compound. Further described are methods and compositions for cancer treatment, for example, by eradicating cancer stem cells.
1. A method for obtaining stem cells from a cell suspension, the method comprising:
providing a cell suspension;
contacting said cell suspension with an antibody specific for Lgr5 so as to bind the antibody specific for Lgr5 to Lgr5 positive cells in the cell suspension; and
at least partially purifying cells bound to the antibody specific for Lgr5 from cells not bound to the antibody specific for Lgr5 from the cell suspension so as to obtain stem cells.
2. The method according to claim 1 , wherein said antibody specific for Lgr5 is an antibody fragment able to bind to Lgr5.
3. The method according to claim 1 , wherein the antibody is labelled.
4. The method according to claim 1 , claim 2 , or claim 3 , wherein said stem cells comprise intestinal, retina, brain, breast, hair follicle, stomach, liver, lung, pancreas, testis, heart or ovarian stem cells.
5. The method according to claim 1 , claim 2 , or claim 3 , wherein at least two different antibodies specific for Lgr5 are contacted with said cell suspension.
6. The method according to claim 1 , wherein at least partially purifying cells bound to the antibody specific for Lgr5 from cells not bound to the antibody specific for Lgr5 comprises using Fluorescence-Activated Cell Sorting.
7. The method according to claim 1 , wherein the cells bound to the antibody specific for Lgr5 are tissue or organ stem cells.
8. The method according to claim 1 , wherein the cell suspension is from a solid or liquid tumor sample; and wherein the stem cells bound to the antibody specific for Lgr5 comprise cancer stem cells.
9. The method according to claim 1 , wherein the antibody specific for Lgr5 comprises a CDR1, CDR2 and/or CDR3 sequence of the light chain sequence of SEQ ID NO:61.
10. The method according to claim 1 , wherein the antibody specific for Lgr5 is a monoclonal antibody or fragment thereof.
11. The method according to claim 1 , wherein the antibody specific for Lgr5 is a chimeric, deimmunized, or humanized monoclonal antibody fragment.
12. The method according to claim 1 , wherein the cell suspension is from a normal tissue or organ sample.
13. The method according to claim 1 , further comprising isolating the stem cells from the antibody specific for Lgr5.
14. A method for isolating tissue or organ stem cells, the method comprising:
utilizing an antibody specific for Lgr5 to identify the tissue or organ stem cells and
isolating the tissue or organ stem cells.