Plant CO
The invention provides compositions and methods for manipulating the exchange of water and/or carbon dioxide (CO 2 ) through plant stomata by controlling CO 2 sensor genes. The invention provides compositions and methods for enhancing or optimizing biomass accumulation in a plant. The invention provides compositions and methods for for opening or closing a stomatal pore on a guard cell in the epidermis of a plant. The invention provides compositions and methods for increasing or decreasing oxygenation efficiency and/or carbon fixation in a guard cell in the epidermis of a plant by manipulating expression of a ribulose-1,5-bisphosphate carboxylase/oxygenase. The invention provides promoters for regulating expression of a nucleic acid in a plant guard cell.
1. A method for down-regulating or decreasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell of a plant, a plant cell, a plant leaf, or a plant part comprising:
(a) transgenically providing to the guard cell of the plant, plant cell, plant leaf, or plant part a nucleic acid or gene comprising:
(i) a nucleic acid encoding a CO 2 Sen (CO 2 sensor) protein; or
(ii) a nucleic acid encoding a polypeptide having a carbonic anhydrase (CA) activity, or a β-carbonic anhydrase activity;
wherein the nucleic acid or gene has at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene, or
the protein or polypeptide is encoded by a nucleic acid or a gene having at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene,
wherein the nucleic acid encodes:
(1) a CO 2 Sen (CO 2 sensor) protein that has a CO 2 Sen (CO 2 sensor) protein activity, and/or
(2) a polypeptide having a carbonic anhydrase or a carbonate dehydratase activity, or a β-carbonic anhydrase activity,
wherein the sequence identity is calculated using a sequence comparison algorithm consisting of a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d “nr pataa” -F F, and all other options are set to default; and
(b) inserting into the guard cell and expressing, or overexpressing, the nucleic acid or gene of (a), thereby down-regulating or decreasing carbon dioxide (CO 2 ) and/or water exchange in the guard cell.
2. A method for regulating water exchange in a guard cell of a plant, a plant cell, a plant leaf, or a plant part comprising:
transgenically inserting into and overexpressing in the guard cell of the plant, plant cell, plant leaf, or plant part a nucleic acid or gene encoding:
(i) a CO 2 Sen (CO 2 sensor) protein, or
(ii) a polypeptide having a carbonic anhydrase (CA) activity, or a 13-carbonic anhydrase activity,
wherein the nucleic acid or gene has at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene, or
the protein or polypeptide is encoded by a nucleic acid or a gene having at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene,
thereby down-regulating or decreasing water exchange in the guard cell.
3. A method for regulating water uptake or water loss in a guard cell of a plant, a plant cell, a plant leaf, or a plant part, comprising transgenically inserting into and overexpressing in the guard cell of the plant, plant cell, plant leaf, or plant part a nucleic acid or gene encoding:
(i) a CO 2 Sen (CO 2 sensor) protein, or
(ii) a polypeptide having a carbonic anhydrase (CA) activity, or a β-carbonic anhydrase activity,
thereby regulating water uptake or water loss,
wherein the nucleic acid or gene has at least about 95% sequence identity to SEQ ID NO:1, or the protein or polypeptide is encoded by a nucleic acid or a gene having at least about 95% sequence identity to SEQ ID NO:1,
wherein overexpressing a CO 2 Sen (CO 2 sensor) or carbonic anhydrase protein in the guard cell decreases water loss.
4. A method for making an enhanced water use efficiency (WUE), or drought-resistant, plant comprising:
transgenically inserting into in a guard cell of the plant and overexpressing or increasing expression of a nucleic acid or a gene encoding:
(i) a CO 2 Sen (CO 2 sensor) protein, or
(ii) a polypeptide having a carbonic anhydrase (CA) activity, or a β-carbonic anhydrase activity;
wherein the nucleic acid or gene has at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene, or
the protein or polypeptide is encoded by a nucleic acid or a gene having at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene,
thereby making an enhanced water use efficiency (WUE), or drought-resistant plant.
5. A plant, a plant part, or a leaf thereof made by the method of claim 4 and comprising said nucleic acid or gene, wherein the plant is:
(i) a dicotyledonous or monocotyledonous plant;
(ii) wheat, oat, rye, barley, rice, sorghum, maize or corn, tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean or soy, a cruciferous plant, a cauliflower, rape or rapa or canola, cane or sugarcane, flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(iii) a specie from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Manihot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
6. A method for closing a stomatal pore on a guard cell in the epidermis of a plant, a guard cell in a plant leaf, or a plant guard cell, comprising transgenically inserting into and overexpressing a nucleic acid or gene encoding:
(a)
(i) a CO 2 Sen (CO 2 sensor) protein, or
(ii) a polypeptide having a carbonic anhydrase (CA) activity, or a 13-carbonic anhydrase activity,
wherein the nucleic acid or gene has at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene, or
the protein or polypeptide is encoded by a nucleic acid or a gene having at least about 95% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene,
thereby causing overexpression and/or increase in expression of the CO 2 Sen protein and/or the carbonic anhydrase (CA), and causing the stomatal pore to close.
7. The method of claim 1 , wherein:
(a) the plant is:
(i) a dicotyledonous or monocotyledonous plant;
(ii) wheat, oat, rye, barley, rice, sorghum, maize or corn, tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean or soy, a cruciferous plant, a cauliflower, rape or rapa or canola, cane or sugarcane, flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(iii) a specie from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Manihot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea;
(b) the plant is characterized by controlled CO 2 exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 , or the plant is characterized by controlled water exchange under ambient 365 ppm CO 2 , elevated ppm CO 2 or reduced ppm CO 2 ; or
(c) the nucleic acid is operably linked to a constitutive or an inducible promoter, a tissue or cell specific promoter, or a promoter responsive to an environmental, a hormonal, a chemical or a developmental signal, or the nucleic acid is operably linked to a plant guard cell-specific promoter.
8. The method of claim 4 , wherein:
(a) the overexpression or increased expression is in a plant guard cell;
(b) the nucleic acid is operably linked to a constitutive or an inducible promoter, a tissue or cell specific promoter, or a promoter responsive to an environmental, a hormonal, a chemical or a developmental signal, or the nucleic acid is operably linked to a plant guard cell-specific promoter;
wherein the sequence identity is calculated using a sequence comparison algorithm consisting of a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d “nr pataa” -F F, and all other options are set to default.
9. The method of claim 7 , wherein:
(a) the plant guard cell-specific promoter comprises a sequence as set forth in SEQ ID NO:10 or SEQ ID NO:11, or functional transcriptional regulatory subsequences thereof, or
(b) the functional or transcriptional regulatory subsequence comprises:
−1140 bp/+23 bp of SEQ ID NO: 10 (pGC1(D1)), −861 bp/+23 bp of SEQ ID NO: 10 (pGC1(D2)) or −443 bp/+23 bp of SEQ ID NO: 10 (pGB1D3)).
10. The method of claim 1 , wherein the sequence identity has at least about 98% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene.
11. The method of claim 10 , wherein the sequence identity is 100%.
12. The method of claim 8 , wherein the sequence identity has at least about 98% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene.
13. The method of claim 12 , wherein the sequence identity is 100%.
14. The method of claim 1 , wherein the sequence identity has at least about 98% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35, over the full length of a protein coding sequence or gene.
15. The method of claim 14 , wherein the sequence identity is 100%.
16. The method of claim 8 , wherein the plant guard cell-specific promoter comprises a sequence as set forth in SEQ ID NO:10 or SEQ ID NO:11, or functional transcriptional regulatory subsequences thereof, or a functional transcriptional regulatory subsequence comprising: −1140 bp/+23 bp of SEQ ID NO: 10 (pGC1(D1)), −861 bp/+23 bp of SEQ ID NO: 10 (pGC1(D2)) or −443 bp/+23 bp of SEQ ID NO: 10 (pGB1D3)).
17. A method for down-regulating or decreasing carbon dioxide (CO 2 ) and/or water exchange in a guard cell of a plant, a plant cell, a plant leaf, or a plant part comprising:
(a) providing to the plant, plant cell, plant leaf, or plant part a nucleic acid comprising:
(i) a nucleotide sequence as set forth in SEQ ID NO:1, or
(ii) a nucleotide sequence encoding a polypeptide comprising a sequence as set forth in SEQ ID NO:3;
(b) inserting into the guard cell, plant cell, plant leaf, or plant part, and expressing, or overexpressing, the nucleic acid of (a),
thereby down-regulating or decreasing carbon dioxide (CO 2 ) and/or water exchange in the guard cell, plant cell, plant leaf, or plant part.
18. A seed derived from the plant of claim 5 , wherein the seed comprises said nucleic acid or gene.
19. The seed of claim 18 , wherein the nucleic acid encoding:
(i) the CO 2 Sen (CO 2 sensor) protein, or
(ii) the polypeptide having a carbonic anhydrase (CA) activity, or β-carbonic anhydrase activity,
is operatively linked to a heterologous promoter.
20. The seed of claim 19 , wherein the heterologous promoter comprises a constitutive or an inducible promoter, a tissue or cell specific promoter, or a promoter responsive to an environmental, a hormonal, a chemical or a developmental signal, a plant guard cell-specific promoter.
21. The seed of claim 20 , wherein the plant guard cell-specific promoter comprises:
(a) a sequence as set forth in SEQ ID NO:10 or SEQ ID NO:11, or functional transcriptional regulatory subsequences thereof, or
(b) the sequence of (a), wherein the functional transcriptional regulatory subsequence comprises:
−1140 bp/+23 bp of SEQ ID NO: 10 (pGC1(D1)), −861 bp/+23 bp of SEQ ID NO: 10 (pGC1(D2)) or −443 bp/+23 bp of SEQ ID NO: 10 (pGB1D3)).